激光免疫疗法对小鼠H22肝癌的长期抗瘤效应

目的观察激光免疫疗法对小鼠H22肝癌的抗瘤效应。方法昆明种小鼠240只,随机分为4组,每组60只。光动力学组：小鼠腹腔内注射HpD10 mg／kg后48 h行激光照射（功率320 W,时间400 s,能量130 J）;激光免疫组：处理同前组,另于照光前12 h小鼠瘤内及瘤周注射糖基化氨基葡糖（GC） 0．2 ml／只;单纯GC组：仅于瘤内及瘤周注射GC;对照组：不做任何处理。比较前3个实验组的抑瘤率、生存率。结果3实验组小鼠均有显著抑瘤效果,激光免疫组的抑瘤率、生存率较单纯免疫佐剂治疗组和光动力学治疗组有显著提高,激光免疫组23．3％肿瘤治愈小鼠长期存活。结论激光免疫疗法对小鼠H22肝癌显示出长期抗瘤效应,该疗法可能成为一种长期有效的综合治疗肿瘤新方法。

Long-term Anti-cancer Effect on H22 Liver Cancer after Laser Photo-immunotherapy

Objective To study the long-term anti-cancer effect on H22 liver cancer after laser photo-immunotherapy in mice. Methods Anti-cancer effect, tumor inhibition rate, survival rate and pathology were observed respectively in immunoadjuvant( glycat-ed chitosan) ,photodynamic therapy ( PDT) and laser photo-immunotherapy ( PDT + glycated chitosan) groups, as well as in control group(n =60 in each group). Results Immunoadjuvant, PDT and laser photo-immunotherapy could inhibit the tumor growth rate effectively compared to the contrast group. The tumor inhibition rate of laser photo-immunotherapy group was 60. 4% which was significantly higher than the immunoadju-vant and PDT groups. 23. 3% of mice of laser photo-immunotherapy group were cured and long - term survivable. Conclusions Laser photo-immunotherapy demonstrates long-term anti-cancer effect on H22 liver cancer which indicates that laser photo-immunotherapy maybe a novel modality for cancer treatment.