骨保护素基因rs2073617T/C、rs2073618G/C多态性在东南沿海地区汉族人群中分布及与急性冠状动脉综合征相关性研究

目的 探讨骨保护素(OPG)基因启动子rs2073617T/C和第一外显子rs2073618G/C位点基因多态性在福建地区汉族人群中的分布及与急性冠状动脉综合征(ACS)的相关性.方法 纳入720例福建地区无血缘关系的汉族人为研究对象,分成ACS 360例(ACS组)和对照组360例(对照组),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对OPG基因rs2073617T/C和rs2073618G/C多态性位点进行基因型分型,同时采用DNA测序对酶切产物进行鉴定.结果 (1)在福建地区汉族人群中,OPG基因rs2073617T/C多态性位点存在TC、TT、CC三种基因型;rs2073618G/C多态性位点也存在GG、GC、CC三种基因型.(2)对ACS组与正常对照组OPG基因rs2073617T/C、rs2073618G/C基因型及等位基因频率分布进行比较均无统计学差异(P均>0.05).(3)ACS组患者单支病变、双支病变及三支以上病变组之间比较OPG基因rs2073617T/C、rs2073618G/C各基因型差异无统计学意义(P均>0.05).结论 福建地区汉族人群OPG rs2073617T/C、rs2073618G/C位点基因多态性与ACS发生无明确相关性.

Correlation between osteoprotegerin gene rs2073617T/C,rs2073618G/C polymorphism and acute coronary syndromes in Fujian area

Objective To study the genotype distribution of osteoprotegerin gene rs2073617T/C and rs2073618G/C polymorphism in Han population in Fujian area and discuss its association with acute coronary syndrome.Methods A total of seven hundreds and twenty consecutive patients with ACS of unrelated Fujian Han people was enrolled in the present study,three hundred and sixty patients with ACS(case group)and 360 matched controls.OPG gene rs2073617T/C and rs2073618G/C were genotyped in all the patients enrolled using polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP).DNA sequences of enzyme digested products were also analyzed.Results (1)OPG rs2073617T/C polymorphism was existing in Hans population of Fujian,TC,TT,CC genotype.OPG rs2073618G/C polymorphism was existing in Hans population of Fujian,GG,GC,CC genotype.(2)No significant differences were observed in the genotype and allelic frequencies of OPG gene rs2073617T/C,rs2073618G/C between the ACS and control groups.(3)ACS patients with single vessel disease,double vessel disease and three or more lesions,OPG gene rs2073617T/C,rs2073618G/C in all lesions had no difference anmoy the groups.Conclusions OPG gene rs2073617T/C,rs2073618G/C polymorphism were not assoiated with acute coronary syndrome in Hans population of Fujian.