Discrepant rotavirus results in two laboratories using the same enzyme immunoassay

Rotavirus testing was performed on 66 stool samples in two separate laboratories using the same enzyme immunoassay. Sixteen of 46 of the positive results reported by the reference laboratory were negative in the hospital laboratory. In addition, RNA gel electrophoresis had negative results in the 16 discrepant samples. This indicates the need to repeat or confirm positive results.