侧柏叶煮散饮片煎煮质量评价

目的：对比侧柏叶煮散饮片与市售原饮片煎煮质量。方法：应用ITS2序列作为DNA条形码对侧柏叶进行分子鉴定，采用化学指纹图谱表征药材化学组成，评价市售饮片及精准煮散饮片的相似度，测定指标成分槲皮苷的含量，标定指纹图谱共有峰。同时，采用标准汤剂煎煮法，比较市售饮片及精准煮散饮片的煎煮效率，对煮散体系进行综合评价。结果：ITS2序列对侧柏叶药材可实现准确鉴定。与原饮片比较，煮散饮片煎煮效率有所增加，且指纹图谱相似度有所提高，但按标准汤剂煎煮法，煮散饮片出膏率增加20%左右，指标性成分槲皮苷的含量未有显著增长，其余化学成分的煎出率也没有明显变化。结论：精准煮散饮片在一定程度上有利于提高侧柏叶的煎煮效率及药材均一性。

Quality Evaluation of the Decoctions of Platycladus cacumen Between the Precise Powder Decoction Pieces and Traditional Chinese Medical(TCM)Slices

This study aimed at comparing the precise powder decoction pieces and market raw TCM slices of P.cacumen over the decocting quality.ITS2 sequence was adopted as a DNA barcode to identify P.cacumen.The chemical composition of the medicinal materials was characterized by HPLC fingerprints for the evaluation of the similarity of precise powder decoction pieces and market TCM slices.The concentrations of quercitrin were determined using UPLC,and the characteristic common peaks were identified.In addition,the extraction efficiency between the market TCM slices and the precise powder decoction pieces was also compared by standard decoction method.It was found that P.cacumen was accurately identified by ITS2 sequences.HPLC fingerprints showed that the extraction efficiency and similarity of the precise powder decoction pieces increased compared with the market TCM slices.However,the extraction yield rate of the precise powder decoction pieces was improved by 20% increased in accordance with the standard decoction method,while the contents of the index component,quercitrin,presented rare increase and the decocting rates of the other chemical components little change in the study.In conclusion,it was indicated that precise powder decoction pieces improved the extraction efficiency and uniformity in comparison with TCM slices.