叶类药材淫羊藿精准煮散饮片的质量研究

目的：采用化学指纹图谱法结合DNA分子鉴定技术，考察淫羊藿精准煮散饮片与市售原饮片质量差异。方法：制备不同规格淫羊藿煮散饮片，对比市售饮片及煮散饮片的干膏收率；应用ITS2及psbA-trnH条形码序列对淫羊藿饮片进行分子鉴定；采用HPLC-DAD法进行3批次饮片混合粉碎前后质量均一性、指纹图谱及相似度评价。结果：淫羊藿煮散饮片出膏率略高于市售饮片；3批市售饮片指标成分淫羊藿苷提取率有明显的差异性，RSD为15.56%，煮散饮片差异较小RSD为6.82%。指纹图谱相似度评价结果显示，淫羊藿精准煮散饮片的指纹图谱相似度提高，标定共有峰10个，峰面积均有提高。ITS2及psbA-trnH序列对淫羊藿煮散饮片可实现准确鉴定。结论：淫羊藿精准煮散饮片与市售饮片基本属性相一致，但煮散饮片的浸膏提取率、指标成分的批间均一性有不同程度提升。研究结果显示发展精准煮散饮片有良好的应用前景。

A Quality Research on the Precise Powder Decoction Pieces of Medicinal Leafage Epimedii Folium

This study aimed at evaluating the quality of precise powder decoction pieces(PPDP)of E.Folium(EF)compared with the traditional commercial slices by chemical fingerprint methods and DNA molecular identification technology.Different specifications of PPDP were prepared,and their dry extract contents were in contrast with that of commercial slices.The slices of EF were identified using ITS2 and psbA-trnH sequences.Three batches of commercial slices were collected,and the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were detected by HPLC-DAD and DNA sequence alignment.It was found that the paste rate of PPDP was slightly higher than that of the traditional commercial slices.The dissolution of chlorogenic acid of PPDP was higher than that of the traditional commercial slices.RSD of inter-assay dissolutions of chlorogenic acid of commercial slices was 15.56%,which was reduced to 6.82% after mixing and preparing into PPDP.The fingerprints showed that the similarity of the PPDP of EF was elevated with the inceases of 10 marketed common peaks.The PPDP of EF was accurately identified by ITS2 and psbAtrnH sequences.In conclusion,compared with tr aditional commercial slices of EF,the PPDP appar ently improved the dissolution rate and the quality uniformity,demonstrated that the boiled powder of CRP achieved obvious clinic advantages.