| 肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定 |
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| 引用本文： | 徐为,顾玉明,高超,郑骏年,郭贞,宋文哲,李海龙,孙晓磊,姚元虎,张宝福,刘俊杰,刘斌.肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定[J].中华实验外科杂志,2006,26(1):892-894. |
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| 作者姓名： | 徐为 顾玉明 高超 郑骏年 郭贞 宋文哲 李海龙 孙晓磊 姚元虎 张宝福 刘俊杰 刘斌 |
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| 作者单位： | 徐州医学院附属医院肿瘤中心,江苏,221002;徐州医学院肿瘤生物治疗重点实验室; |
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| 摘 要： | Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.
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| 关 键 词： | Ki-67抗原 细胞毒性T淋巴细胞 表位 |
Identification of HLA-A2-restricted CTL epitopes encoded by Ki-67 antigen |
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| Abstract: | |
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| Keywords: | Ki-67 antigenCTLEpitope |
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