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| 流式细胞术在人急性白血病免疫分型中的应用 | |
| 引用本文： | 邹典斌,张秋堂,郭荣,魏国庆,何飞. 流式细胞术在人急性白血病免疫分型中的应用[J]. 郑州大学学报(医学版), 2001, 36(1): 56-58 |
| 作者姓名： | 邹典斌 张秋堂 郭荣 魏国庆 何飞 |
| 作者单位： | 1. 河南医科大学第一附属医院血液科 郑州 450052 2. 河南医科大学研究生 郑州 450052 |
| 摘 要： | 目的 :研究流式细胞仪 (Flowcytometry,FCM)检测急性白血病 (AL)细胞表面抗原的方法及临床意义。方法 :以FCM为基础 ,应用一组三色荧光标记单克隆抗体 (Monocolonalantibody,MoAb)对 14例AL患者进行免疫分型。结果 :5例为双表型 ,即同时表达二系抗原 (急性髓细胞白血病 (AML) 3例 ,急性淋巴细胞白血病 (ALL) 2例 ) ;9例为系列专一性 (即仅表达一系抗原 ,AML 6例 ,ALL 3例 )。结论 :双表型较系列专一型预后差 ;FCM法具有测定细胞量大 ,快速和准确的优点 ,值得推广应用 |
| 关 键 词： | 白血病 急性 免疫分型 双表型 流式细胞术 |
| 修稿时间： | 2000-05-08 |
Flow cytometry used in the immunophenotype in adult acute leukemia |
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| ZOU Dianbin ),GUO Rong ),ZHANG Qiutang ),WEI Guoqing ),HE Fei ) ). Flow cytometry used in the immunophenotype in adult acute leukemia[J]. Journal of Zhengzhou University: Med Sci, 2001, 36(1): 56-58 | |
| Authors: | ZOU Dianbin ) GUO Rong ) ZHANG Qiutang ) WEI Guoqing ) HE Fei ) ) |
| Affiliation: | ZOU Dianbin 1),GUO Rong 2),ZHANG Qiutang 1),WEI Guoqing 2),HE Fei 2) 1)Department of Hematology,the First Affiliated Hospital,Henan Medical University,Zhengzhou 450052 2)Postgraduate,Henan Mechical University,Zhengzhou 450052 |
| Abstract: | Aim:To study antigen expression characteristic of acute leukemia(AL) immunophenotype by FCM and clinical significance.Methods: Fourteen patients with AL were investigated with MoAb and immunofluorescence.Results: Five cases were characteristic of double lineage expression,namely two lineage antigen expression simultaneously(AML 3 cases,ALL 2 cases);9 cases were characteristic of one lineage expression(AML 5 cases,ALL 4 cases).Conclusion: ALL with double lineage expression is related to poor prognosis;FCM have a characteristic of detecting large quantity of cells,rapidness and accuracy. |
| Keywords: | Leukemia acute Immunophenotype double lineage expression flow cytometry |
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