银丹心脑通软胶囊对血管内皮细胞的保护作用

目的研究银丹心脑通软胶囊（YXC）对血管内皮细胞的保护作用及作用机制。方法采用氧化低密度脂蛋白（Ox-LDL）和肿瘤坏死因子α（TNF-α）诱导人脐静脉内皮细胞（HUVEC）损伤两种模型,MTT法测定YXC预处理后HUVEC存活率,探讨YXC对内皮细胞的保护作用;采用流式细胞术测定YXC对内皮细胞凋亡的影响,探讨YXC内皮保护的作用机制。结果 YXC的最大无毒浓度为500μg·m L^-1。将250、125、62.5μg·m L^-13个浓度作为YXC的实验浓度;YXC孵育24 h后可明显升高Ox-LDL诱导的HUVEC的存活率（P〈0.05）,具有明显的量效关系;YXC孵育24 h后可明显升高TNF-α诱导的HUVEC的存活率（P〈0.05）。250和125μg·m L^-1YXC可显著降低HUVEC细胞的凋亡率。结论 YXC对血管内皮细胞具有保护作用,其机制可能与抗细胞凋亡有关。

Protective Effect of Yindan Xinnaotong Capsule on Endothelial Cells in Vitro

Objective To study the protective effect ofYindan Xinnaotong capsule （YXC） on endothelial cells. Methods Human umbilical vein endothelial cells （HUVECs） were cultured in viro and HUVECs were pretreated with 250 μg·mL^-1, 125 μg·mL^-1 and 62.5 μg·mL^-1 for 24 h. 200 μg·mL^-1 α-LDL and 60 ng·mL^-1 TNF-α were used to induce HUVECs injury. The cell survival rate was determined by MTT, and flow cytometry was used to detect apoptosis to evaluate the mechanism of protective function of YXC. Results The maximal non-cytotoxic concentration of YXC was 500 μg·mL^-1, thus 250, 125 and 62.5μg·mL^-1 were employed as the experimental concentrations. YXC could obviously increase the survival rate of HUVECs induced by Ox-LDL （P 〈0.05） and TNF-ot with a dose-dependence manner; compared with model group, 62.5 and 250 μg·mL^-1 YXC treatment decreased apoptosis rate induced by Ox-LDL HUVECs （P 〈0.05）. Meanwhile, 250 and 125 μg·mL^-1 YXC obviously reduced apoptosis rate induced by TNF-α （P 〈0.05）. Conclusion YXC could protect the HUVEC injury induced by Ox-LDL and TNF-α, and this may be associated with the inhibitory effect on cell apoptosis.