HPLC法双波长同时测定赤芍中4种成分的含量 |
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引用本文: | 柳梦婷,;方婧,;孙昊,;吴宏伟,;付梅红,;杨洪军. HPLC法双波长同时测定赤芍中4种成分的含量[J]. 中国药物警戒, 2014, 0(9): 524-527 |
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作者姓名: | 柳梦婷, 方婧, 孙昊, 吴宏伟, 付梅红, 杨洪军 |
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作者单位: | [1]中国中医科学院中药研究所,北京100700; [2]首都医科大学中医药学院,北京100069 |
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基金项目: | 国家重大新药创制专项:中药大品种脑心通胶囊技术改造研究,课题编号:2011ZX09201-201-25. |
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摘 要: | 目的:采用高效液相色谱法,在不同检测波长下同时测定赤芍中儿茶素、芍药苷、苯甲酸、丹皮酚4种成分的含量。方法色谱柱为DiamonsilC185μm250×4.6mm,以0.1%甲酸-乙腈为流动相进行梯度洗脱,检测波长为230nm(儿茶素、芍药苷、苯甲酸),275nm(丹皮酚),流速1mL·min-1,柱温30℃。结果不同产地赤芍中4种成分含量差异较大,7批不同产地赤芍药材中儿茶素、芍药苷、苯甲酸和丹皮酚的含量范围分别为:0%-0.052%、1.044%-4.326%、0.126%-0.249%、0.022%-1.060%。结论本法操作简便、准确、重复性好,可用于赤芍药材中儿茶素、芍药苷、苯甲酸、丹皮酚含量的测定。
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关 键 词: | 赤芍 高效液相色谱法 儿茶素 芍药苷 苯甲酸 丹皮酚 含量测定 |
HPLC Ddouble Wavelength Simultaneous Determination of Four Components in Paeonia lactiflora Pall |
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Affiliation: | LIU Meng-ting, FANG Jing, SUN Hao, WU Hong-wei, FU Mei-hong, YANG Hong-jun (1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 2Capital Medical University School of Traditional Chinese Medicine, Beijing 100069, China) |
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Abstract: | Objective To establish a HPLC method for determining the content of catechin, paeoniflorin, benzoic acid and paeonol in Paeonia lactiflora Pall at different wavelength. Methods It was performed on Diamonsil colunm C18 μm 250×4.6 mm, mobile phase was 0.1%methanoic acid-methyl cyanide, gradient elute, detection wavelength was 230 nm (catechin, paeoniflorin, benzoic acid) and 275 nm (paeonol), flow rate was 1mL·min-1, and colunm temperature was 30℃. Results The contents of four active components in Paeonia Lactiflora Pall from different habitats were quite different. The contents of catechin, paeoniflorin, benzoic acid and paeonol were 0%-0.052%, 1.044-4.326%, 0.126-0.249%, 0.022-1.060%respectively. Conclusion The method is simple and accurate with good reproducibility, it is suitable for determining the contents of catechin, paeoniflorin, benzoic acid and paeonol in Paeonia lactiflora Pall. |
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Keywords: | Paeonia lactiflora Pall HPLC catechin paeoniflorin benzoic acid paeonol quality control |
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