细胞保护蛋白在人体移植肾中的表达

目的 研究细胞保护蛋白在人体移植肾中的表达情况和临床意义. 方法采用免疫组化染色技术检测人体移植肾急性排斥反应(AR)、慢性排斥反应(CR)和无排斥反应 (NR)3组各10例中细胞保护蛋白A20、血红素加氧酶-1(HO-1)和B细胞淋巴瘤/白血病-XL蛋白(Bcl-XL)的表达情况. 结果 A20、HO-1和Bcl-XL主要表达于内皮细胞、平滑肌细胞和浸润性单核细胞.A20在AR中表达强于CR[(5.04±0.71)vs(3.20±0.64),P＜0.01];A20在NR中不表达.HO-1在AR中表达(7.91±2.24),在CR和NR中不表达.Bcl-XL在3组中均表达(AR:10.62±3.17;CR:8.50±2.45;NR:11.03±2.77),但在CR中表达较弱;AR、 NR与CR相比,差异均具有统计学意义(P＜0.05);而AR组和NR组间的差异无统计学意义. 结论 A20和HO-1可能在AR中发挥细胞保护作用;而在CR中,A20可能是发挥保护作用的主要蛋白.

Expressions of Cytoprotective Proteins for Human Renal Allografts

OBJECTIVE: To investigate the expression status and clinical significance of cytoprotective proteins to human renal allografts. METHODS: Expressions of cytoprotective proteins: antiapoptotic protein (A20), hemeoxygenase-1 (HO-1), and B-cell lymphoma/leukemia-X(L) (Bcl-X(L)) were analyzed by immunohistochemistry and compared in 30 renal allografts, including 10 grafts undergoing acute rejection (AR), 10 grafts undergoing chronic rejection (CR), and 10 nonrejecting (NR) grafts. RESULTS: Expressions of A20, HO-1 and Bcl-X(L) localized mainly in endothelial, smooth muscle, and infiltrating mononuclear cells. A20 expressed in grafts undergoing AR (5.04 +/- 0.71) and CR (3.20 +/- 0.64), not in NR grafts, and its expression in CR was weaker than that in AR ( P < 0.01). HO-1 expressed in AR (7.91 +/- 2.24), not in CR and NR. Bcl-X(L) was detected in all grafts (AR: 10.62 +/- 3.17; CR: 8.50 +/- 2.45; NR: 11.03 +/- 2.77), but had a decreased expression levels in CR. CONCLUSION: A20 and HO-1 may play protective role for AR, and A20 may be the essential protein having protective function for CR.