血管性血友病因子在ABO血型个体中质和量差异的实验研究

目的比较血管性血友病因子在ABO血型中质和量的差异。方法运用ELISA法同时测定A血型、B血型、AB血型及O血型四组正常人的vWF抗原水平(vWF:Ag)、VWF瑞斯托霉素辅因子活性(vWF:Rcof)及vWF胶原结合试验(vWF:CBA),并应用统计方法比较不同血型之间结果的差异。结果O型血个体其vWF:Ag、vWF:Rcof及vWF:CBA均低于非O型血,差异有显著统计学意义(P<0.001);同时,vWF:CBA下降水平大于vWF:Rcof,使得vWF:CBA/Ag(P<0.001)小于vWF:Rcof/Ag(P值为0.84)。结论O型血较非O型血个体有更高的出血危险,在临床诊断vWD的过程中要充分考虑ABO血型系统对血浆vWF水平的影响。

Differences of Quality and Quantity of the von Willebrand Factor on the ABO Blood Group in Laboratory Parameters

Objective To compare the difference of quality and quantity of the von Willebrand factor on the ABO blood group.Methods We detect the levels of von Willebrand factor(vWF) antigen,vWF:ristocetin cofactor,vWF:collagen binding assay by ELISA in 42 blood group O and 101 non-group O blood donors who well-matched for sex and age distribution.Then we compare the differences of the results between the four blood groups by statistics method.Results There are conspicuous differences of vWF:Ag,vWF: Rcof and vWF:CBA between the O group individuals(P<0.001)and non-O group.And reduction in vWF:CBA is greater than vWF:Rcof in O group donors.This lead to a lower vWF: CBA/Ag ratio(P<0.001) in O group donors whereas the vWF: Rcof/ Ag ratio(P=0.84) is unaffected.Conclusions The results showed that the O group individuals have a greater risk for bleeding than non-O group,we must consider the influence of ABO blood group to the plasma levels of vWF during the vWD clinical diagnosis.