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| 卵巢癌组织AM表达及其对ERK活性影响的观察 | |
| 引用本文： | 伍丹丹,张颐,孟祥凯,陈盼,庞晓燕. 卵巢癌组织AM表达及其对ERK活性影响的观察[J]. 中华肿瘤防治杂志, 2011, 18(16): 1262-1266 |
| 作者姓名： | 伍丹丹 张颐 孟祥凯 陈盼 庞晓燕 |
| 作者单位： | 1. 福建医科大学附属泉州市第一医院妇产科,福建泉州,362000 2. 中国医科大学附属第一医院妇科,辽宁沈阳,110001 |
| 基金项目： | 沈阳市科技局课题(1081234-1-00); 辽宁省科技厅社会发展攻关计划课题(2009225035); 辽宁省教育厅课题(2009A775) |
| 摘 要： | 目的:研究卵巢上皮性恶性肿瘤中肾上腺髓质素(AM)的表达及其对ERK活性的影响,探讨其作用机制及临床意义。方法:应用RT-PCR、MTT比色法和蛋白质印迹法对卵巢上皮性恶性肿瘤(30例)、卵巢良性肿瘤(10例)和正常卵巢组织(10例)的AM表达情况及AM、AM受体阻断剂(AM22-52)对卵巢癌细胞增殖和ERK活性的影响进行分析。结果:AM在卵巢上皮性恶性肿瘤中的半定量结果为0.948±0.150,与正常卵巢及卵巢良性肿瘤比较,差异有统计学意义,P=0.042。其表达量与术后病理分级相关,与手术病理分期无关;MTT法检测显示,AM能促进卵巢癌细胞增殖的作用,随着作用浓度和作用时间的增加这种促进作用也随之增强;蛋白质印迹法检测,随着AM作用浓度的增加,磷酸化的ERK1/2的表达随之增高,反之,随着AM受体阻断剂(AM22-52)浓度的增加,磷酸化的ERK1/2的表达随之降低,差异有统计学意义,P<0.05。结论:卵巢癌组织中AM高表达,其能通过MAPK/ERK信号转导途径在卵巢癌的发生中起作用。 |
| 关 键 词： | 肾上腺髓质素 卵巢肿瘤 ERK 细胞增殖 |
Expression of adrenomedullin in ovarian cancer and its influence to ERK activity |
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| WU Dan-dan,ZHANG Yi,MENG Xiang-kai,CHEN Pan,PANG Xiao-yan. Expression of adrenomedullin in ovarian cancer and its influence to ERK activity[J]. Chinese Journal of Cancer Prevention and Treatment, 2011, 18(16): 1262-1266 | |
| Authors: | WU Dan-dan ZHANG Yi MENG Xiang-kai CHEN Pan PANG Xiao-yan |
| Affiliation: | WU Dan-dan1,ZHANG Yi2,MENG Xiang-kai2,CHEN Pan2,PANG Xiao-yan2 1.Department of Gynecology and Obstetrics,First Hospital of Quanzhou,Fujian Medical University,Quanzhou 362000,P.R.China 2.Department of Gynecology,First Affiliated Hospital of China Medical University,Shenyang 110001,P.R.China |
| Abstract: | OBJECTIVE: To evaluate the expression level of AM in epithelial ovarian cancer and its influence to ERK activity,and detect the interaction mechanisms and clinical significance.METHODS: By using RT-PCR,MTT and Western blot the expression level of AM in 10 cases normal ovarian tissues,10cases benign ovarian epithelial tumors,and 30 cases malignant ovarian epithelial tumors were analyzed,and the influence of different density AM and the AM acceptor blocking agent(AM22-52) on the ovarian cancer cell proliferat... |
| Keywords: | adrenomedullin ovarian neoplasms ERK cell proliferation |
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