溃疡性结肠炎结肠黏膜修复中HGF,c-Met,EGFR的作用

目的:观察并分析肝细胞生长因子(HGF)及其受体c-Met与表皮生长因子受体(EGFR)在溃疡性结肠炎(UC)活动和非活动阶段患者结肠黏膜的表达情况,探讨其表达的临床意义．方法:根据改良Williams疾病活动指数(DAI) 将42例UC患者分为活动期(n=25)和非活动期(n=17)2组,对照组(n=20)为门诊健康体检者或肠易激综合征患者．结肠镜下活检各组患者结肠黏膜组织,采用免疫组化SABC法检测各组患者结肠黏膜HGF及c-Met表达;SP法检测EGFR及增殖细胞核抗原(PCNA)表达．结果:对照组、活动期UC患者、非活动期 UC患者HGF阳性表达率分别为22％,88％, 100％(X2=62．84,P<0．01);c-Met阳性表达率分别为25％,92％,100％(X2=62．34,P<0．01); EGFR阳性表达率分别为25％,92％,1 00％(X2 =54．34,P<0．01);PCNA过表达率分别为0, 36％,100％(X2=67．50,P<0．01),组间比较差异显著．HGF,c-Met和EGFR在UC患者结肠黏膜表达与PCNA过表达正相关(r=0．648,0．645, 0．565,P<0．01)．结论:HGF,c-Met,EGFR及PCNA在非活动期UC患者结肠黏膜中的表达较活动期UC患者和对照组明显增加．HGF及其受体c-Met与 EGFR在UC患者结肠炎症黏膜修复过程中可能起一定作用．

Roles of hepatocyte growth factor, c-Met and epidermal growth factor receptor in repair of colonic mucosa from patients with ulcerative colitis

AIM: To investigate the expression of hepatocyte growth factor (HGF), c-Met and epidermal growth factor receptor (EGFR) in colonic mucosa from patients with active or inactive ulcerative colitis and their clinical significances. METHODS: Forty-two patients with ulcerative colitis were divided into 2 groups (active: n = 25; inactive: n = 17) according to the modified Williams Disease Activity Index (DAI). Health examinees from outpatient department or patients hospitalized for irr-itable bowel syndrome were selected as controls. All colon-ic mucosa specimens were obtained from colonoscopic examination. SABC immuno-histochemistry was used to evaluate the expression of hepatocyte growth factor and c-Met, and SP immunohistochemistry was used to evaluate the expression of epidermal growth factor receptor and proliferating cell nuclear antigen. RESULTS: In control group, active and inactive ulcerative colitis group, the positive rates of HGF expression were 25%, 88% and 100%, respectively, and those of c-Met expression were 25%, 92% and 100%, respectively. There were significant differences among the above three groups (HGF: X2 = 62.84, P < 0.01; c-Met: X2 = 62.34, P < 0.01). The expression of EGFR in control group, active and inactive ulcerative colitis group were 25%, 92% and 100%, respect-ively, and those of PCNA were 0, 36%, and 100%, respectively. There also existed significant differences among the above three groups (X2 = 54.34, P < 0.01; X2 = 67.50, P <0.01). In addition, the expression of HGF, c-Met and EGFR in ulcerative colitis were significantly correlated with the over-expression of PCNA (r = 0.648, P < 0.01; r = 0.645, P < 0.01; r = 0.565, P < 0.01, respectively). CONCLUSION: HGF, c-Met and EGFR may be involved in the repair process of inflamed mucosa in ulcerative colitis.