嗅鞘细胞对脊髓源性神经干细胞分化的影响

目的 探讨嗅鞘细胞对脊髓源性神经干细胞分化的作用。方法 应用Millicell插入式细胞培养皿，大鼠脊髓源性神经干细胞与嗅鞘细胞在无血清培养液中共培养，通过免疫化学染色方法，检测脊髓源性神经干细胞分化为神经元、星形胶质细胞和少突胶质细胞的比例。结果 共培养7d后，脊髓源性神经干细胞分化为神经元的比例高达43．5％，明显高于对照组的21．4％。结论 嗅鞘细胞提供的微环境可促进脊髓源性神经干细胞分化为神经元。

Effect of olfactory ensheathing cells on differentiation of spinal cord-derived neural stem cells

Objective To investigate the role of olfactory ensheathing cells in differentiating spinal cord-derived neural stem cells(NSCs).Methods By using Millicell cell culture plate insert,rat spinal cord-derived NSCs were co-cultured with olfactory ensheathing cells in the serum-free medium.The proportion of neurons,astrocytes and oligodendrocytes differentiated by spinal cord-derived NSCs was investigated by means of immunochemical staining.Results At day 7 of co-culture,the proportion of spinal cord-derived NSCs differentiating into neurons was as high as 43.5%,which was significantly higher than 21.4% in control group.Conclusions The environmental cues provided by olfactory ensheathing cells can promote the differentiation of co-cultured spinal cord-derived NSCs into neurons.