Typing of sandflies from Greece and Cyprus by DNA polymorphism of 18S rRNA gene |
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| Authors: | A. M. Aransay E. Scoulica B. Chaniotis Y. Tselentis |
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| Affiliation: | University of Crete, Faculty of Medicine, Laboratory of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, WHO Collaborating Centre for Research and Training in Mediterranean Zoonoses, PO Box 1393, 71409 Heraklion, Crete, Greece |
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| Abstract: | A simple and reliable technique was developed to distinguish Phlebotomine sandflies by restriction fragment length polymorphism of PCR-amplified (PCR-RFLP) 18S rDNAs. Seven morphologically identified sandflies species from several localities of Greece and Cyprus were studied, and specific patterns were developed by double digesting amplified 18S rDNAs with Hpall and Rsal. Three additional species of the sub-genus Larroussius were distinguished by a second double digestion with Accl and Banl. We have successfully applied the method on samples in which morphological characters were badly distinguished due to poor storage conditions and in larval stages. |
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| Keywords: | PCR-RFLP Phlebotomine sandflies typing 18S ribosomal DNA |
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