| 熟地强筋合剂对大鼠骨髓基质细胞向成骨细胞分化的影响 |
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| 引用本文： | 孙克民,王平,卢启贵,黄东红,裴代平.熟地强筋合剂对大鼠骨髓基质细胞向成骨细胞分化的影响[J].中国医药导刊,2011,13(11):1938-1939. |
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| 作者姓名： | 孙克民 王平 卢启贵 黄东红 裴代平 |
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| 作者单位： | 广东省深圳平乐骨伤科医院,深圳,518010 |
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| 摘 要： | 目的:观察熟地强筋合剂在骨髓基质细胞向成骨分化过程中的作用。方法:采用体外培养大鼠骨髓基质细胞,分别观察各含药血清组碱性磷酸酶染色、Ⅰ型胶原染色情况;取各组不同时间点培养液测定碱性磷酸酶比活性及骨钙素含量。结果:碱性磷酸酶染色强弱顺序为D组>C组>B组,Ⅰ型胶原染色强弱顺序为D组>C组>B组>A组,碱性磷酸酶比活性各加药组与对照组比较明显提高(P<0.05);各加药组与对照组比较BGP含量均明显提高。结论:熟地强筋合剂能促进大鼠骨髓基质细胞增殖,诱导大鼠骨髓基质细胞向成骨细胞分化。
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| 关 键 词： | 熟地强筋合剂 骨髓基质细胞 成骨细胞 诱导分化 |
The Effect on Differentiation to Osteoblast of SD Rat'S Bone Marrow Stromal Cells in vitro by Shu Di Qiang Jin Mixture |
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| Institution: | Sun Ke-min,Wang Ping,Lu Qi-gui,et al. (Shenzhen Pingle Orthopaedic Hospital,Guangdong Province,Shenzhen 518010,China) |
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| Abstract: | Objective:To observe the differentiation to osteoblast of SD rat’ s marrow stromal cell cultured by Shu Di Qiang Jin Mixture.Methods:The serum pharmacology method was used to culture the SD rat’ s marrow stromal cell.The alizarin Bordeaux starting,Van-GiegonⅠcollagen staining method were used to observe the expressions of ALP and typeⅠcollagen respectively on different collection time.Results:The intensity of ALP dyeing sequenced as:D>C>B,and collagenⅠdyeing sequenced as:D>C>B>A.The alkaline phosphatase activity and the amount of bone glaprotein were higher in B,C,D groups compared with the control group at the same time(P<0,05).Conclusion:Shu Di Qiang Jin Mixture could accelerate the proliferation of the BMSCs,and could induce the BMSCs differentiation,accelerate the proliferation and differentiation of the BMSCs. |
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| Keywords: | Shu Di Qiang Jin Mixture BMSCs Osteoblasts Differentiation |
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