| 补阳还五汤对体外培养的大鼠皮层神经元缺氧凋亡的影响 |
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| 引用本文： | 曲宏达,佟丽,沈剑刚,陈育尧. 补阳还五汤对体外培养的大鼠皮层神经元缺氧凋亡的影响[J]. 南方医科大学学报, 2002, 22(1): 35-38 |
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| 作者姓名： | 曲宏达 佟丽 沈剑刚 陈育尧 |
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| 作者单位： | 第一军医大学中医系,广东,广州,510515;第一军医大学中医系,广东,广州,510515;第一军医大学中医系,广东,广州,510515;第一军医大学中医系,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金(39970900) |
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| 摘 要： | 目的探讨补阳还五汤对神经元缺氧凋亡的作用,以及对神经元缺氧过程氧自由基(OFR)、一氧化氮(NO)生成和bcl-2基因表达的影响。方法采用Daniel方法建立神经元缺氧凋亡模型,加入补阳还五汤药物血清,应用碘化丙啶(propidiumiodide,PI)染色法经流式细胞仪检测神经元凋亡率,分光光度法检测丙二醛(MDA)、NO浓度,免疫组化法检测bcl-2表达。结果补阳还五汤显著抑制缺氧导致的神经元凋亡,并减少神经元缺氧过程NO、OFR的生成,上调bcl-2基因的表达。结论补阳还五汤对神经元缺氧凋亡有抑制作用,其机制可能与其减少神经元缺氧过程NO、OFR生成,及上调bcl-2基因表达有关。
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| 关 键 词： | 补阳还五汤 神经元 凋亡 bcl-2 |
| 文章编号： | 1000-2588(2002)01-0035-04 |
| 修稿时间： | 2001-06-04 |
Protective effect of Buyanghuanwu Tang on cultured rat cortical neurons against hypoxia-induced apoptosis |
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| QUHong-da,TONGLi,SHENJian-gang,CHENYu-yao Departmentof TraditionalChineseMedicine,FirstMilitaryMedicalUniversity,Guangzhou,China. Protective effect of Buyanghuanwu Tang on cultured rat cortical neurons against hypoxia-induced apoptosis[J]. Journal of Southern Medical University, 2002, 22(1): 35-38 |
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| Authors: | QUHong-da TONGLi SHENJian-gang CHENYu-yao Departmentof TraditionalChineseMedicine FirstMilitaryMedicalUniversity Guangzhou China |
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| Affiliation: | QUHong-da,TONGLi,SHENJian-gang,CHENYu-yao Departmentof TraditionalChineseMedicine,FirstMilitaryMedicalUniversity,Guangzhou510515,China |
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| Abstract: | Objective To investigatetheprotectiveeffectof BuyanghuanwuTang(BYHWT),a decoctionwith6herbal ingredients,on in vitro culturedratcorticalneuronsagainstapoptosisinducedby hypoxia,and studyitseffecton the productionof nitricoxide(NO) andoxygenfreeradicalsandBcl-2expressionin theneuronsduringhypoxia.Methods Modelsof hypoxia-inducedneuronapoptosiswere establishedand treatedwithsera containingBYHWT.Stainedby propidiumiodide,theneuronsunderwentapoptosisanalysisusingflowcytometry,andthelevelsof malonyldialdehyde(MDA) and NO weremeasuredwithspectrophotometer,withBcl-2expressionassayedby immunohistochemicalmethod withflowcytometry.Results In BYHWT-treatedneurons,apoptosisratesweresignificantlylowerthanthoseof theneurons subjectedto hypoxiaexclusively,andat thesametimeNO andMDAproductionwas remarkablyreduced.Bcl-2expression,however,wasup-regulated.Conclusion BYHWTcanprotectneuronsfromhypoxia-inducedapoptosis,themechanismof whichmay lie in the eliminationof NO and oxygenfreeradicalsproducedduringhypoxiaand up-regulationof Bcl-2expression. |
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| Keywords: | traditionalChinesemedicine neuron apotosis bcl-2 |
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