肉桂油治疗小鼠CVB3m病毒性心肌炎的实验研究

目的:研究肉桂油(OC)对柯萨奇病毒B3m(CVB3m)诱发性小鼠病毒性心肌炎(VMC)的治疗作用.方法:0.1 mL CVB3m ip建立BALB/c小鼠VMC模型.正常对照组同法接种0.1 mL不含病毒的Eagle液,与感染病毒小鼠隔离饲养.于接种病毒72 h后给药,OC治疗组(49.1,36.7,26.5 mg/kg肉桂油,ig)、阳性药物组(50 mg/kg黄芪注射液,ip)、模型组与正常对照组(2500 mg/kg生理盐水,ig),连续给药1 wk.观察指标:累计死亡率与中位生存时间;接种病毒后10 d心肌组织光、电镜组织病理学检查;心肌匀浆MDA含量、SOD活性;血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)含量;21 d心肌组织光镜病理检查.结果:OC 49.1和36.7 mg/kg治疗组可降低小鼠死亡率,延长中位生存时间,降低急性期血清中CK,CK-MB含量以及心肌中MDA含量,提高SOD活性;减轻急性期、亚急性期小鼠心肌组织的坏死与钙化,与模型组比较差异显著(P<0.05).结论:OC具有治疗柯萨奇病毒B3m诱发性小鼠VMC的作用.

Effect of Oleum Cinnamomi on mice viral myocarditis caused by CVB3m

AIM: To investigate the therapeutic effect of Oleum Cinnamomi(OC), volatile oil of cassia bark, on mice viral myocarditis caused by coxsackie virus B 3m(CVB 3m). METHODS: Male BALB/c mice were randomly divided into 6 groups: 1 control group (n=16)treated with normal saline i.p, 1 model group (n=30) treated with normal saline, 1 RA group (n=30) treated with 50 mg/kg Radix astragali injection i.p. as positive control and 3 treatment groups (30 in each group) respectively given 49.1, 36.7 and 26.5 mg/kg OC after 72 h viral inoculation. Except for the control group, the other 5 groups were all inoculated with CVB 3m i.p. and were bred in insulation lab. After one week treatment, the death rate, the heart histopathologic changes, the content of CK, CK-MB and LDH in mice serum, and MAD and SOD in myocardium were observed. RESULTS: The death rate, the histopathologic score, the content of CK and CK-MB in mice serum and MAD in myocardium were lower and the activities of SOD were higher in 49.1 and 36.7 mg/kg OC groups than in model group (P<0.05). The death rate was lower (P<0.05) and the histopathologic changes (necrosis, degeneration and cellular infiltration) were milder in OC groups than in model group at 21 d after viral inoculation. CONCLUSION: OC has a significant therapeutic effect on treatment of viral myocarditis caused by CVB 3m.