羟基磷灰石/聚苯硫醚复合材料的制备及体外生物相容性

背景：利用传统固相复合工艺难以将纳米级羟基磷灰石粉体与聚苯硫醚进行复合，从而无法发挥纳米材料的优势，并且目前缺少羟基磷灰石/聚苯硫醚复合材料生物相容性的相关研究。目的：应用液相复合工艺制备纳米羟基磷灰石/聚苯硫醚复合材料，评价其体外生物相容性。方法：应用液相复合工艺制备纳米羟基磷灰石/聚苯硫醚复合材料粉体和片材，利用红外光谱分析仪及扫描电镜分析复合材料的成分结构；利用接触角测试仪评估材料的亲水性，利用兔血及L929小鼠成纤维细胞进行材料的溶血实验及体外细胞毒性实验。结果与结论：通过液相复合工艺制备的羟基磷灰石/聚苯硫醚复合材料混合均匀，结合紧密；聚苯硫醚粉体的接触角为81.25＆#176;，而纳米羟基磷灰石/聚苯硫醚复合材料粉体接触角为16.1＆#176;；聚苯硫醚片材的接触角为83.5＆#176;，纳米羟基磷灰石/聚苯硫醚复合材料片材的接触角为42.5＆#176;，对比可知，纳米羟基磷灰石的加入明显改善了单纯聚苯硫醚材料的亲水性能。纳米羟基磷灰石/聚苯硫醚复合材料的溶血率为1.66%（〈5%），提示材料血液相容性良好；L929细胞在材料浸提液中生长良好，CCK-8法检测提示体外细胞毒性属无毒范畴（0至1级）。以上结果表明羟基磷灰石/聚苯硫醚复合材料制备成功，体外生物相容性良好。

Preparation andin vitro biocompatibility of hydroxyapatite/polyphenylene sulfide composites

BACKGROUND： It is difficult to combine nano-hydroxyapatite with polyphenylene sulfide by traditional method, thus which is unable to take advantage of nanomaterials. There is still a lack of the research of biocompatibility of nano-hydroxyapatite/polyphenylene sulfide composites.
OBJECTIVE： To prepare the nano-hydroxyapatite/polyphenylene sulfide composites and to evaluate the in vitro biocompatibility.
METHODS： Liquid composite technology was applied to prepare the nano-hydroxyapatit/polyphenylene sulfide composites; infrared spectrometer and scanning electron microscope were applied to analyze the composite constituent structure. Contact angle test, hemolysis test and cytotoxicity test in vitro were conducted with contact angle meter, cony blood and L929 mouse fibroblast cells.
RESULTS AND CONCLUSION： The nano-hydroxyapatite/polyphenylene sulfide composites were intermixed evenly and closely; the composites owned good hydrophilic performance （sheet： 42.5°, powder： 16.1°）. The hemolysis ratio of nano-hydroxyapatite/polyphenylene sulfide composites was less than 5% （1.66%）, which showed good blood compatibility. L929 cells grew well in the leaching liquor of the composites. The cell counting kit-8 test showed the cytotoxicity in vitro belongs to non-toxic category （level 0-1）. All these results prove that the nano-hydroxyapatite/polyphenylene sulfide composites have been prepared successfully and show excellent histocompatibility in vitro.