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高效液相色谱法测定银翘解毒颗粒中绿原酸的含量
引用本文:李成网. 高效液相色谱法测定银翘解毒颗粒中绿原酸的含量[J]. 中国实验方剂学杂志, 2004, 10(5): 3-4
作者姓名:李成网
作者单位:安徽省医学科学研究所,安徽,合肥,230061
基金项目:安徽省优秀青年科技基金 (0 4 0 4 30 55)
摘    要:
目的:运用高效液相色谱法测定银翘解毒颗粒中绿原酸的含量。方法:采用HypersilC18柱,甲醇-水-冰醋酸-三乙胺(15:85:1:0.2)为流动相,检测波长为324nm。结果:绿原酸的回收率为98.12%,RSD=0.97%(n=5)。结论:本法简便、快速、重复性好,可作为制剂的定量分析方法。

关 键 词:绿原酸  银翘解毒颗粒  高效液相色谱
文章编号:1005-9903(2004)05-0003-02
收稿时间:2003-09-11
修稿时间:2003-09-11

Determination of Chlorogenic Acid in Yinqiaojiedu Granuces by HPLC
LI Cheng-wang. Determination of Chlorogenic Acid in Yinqiaojiedu Granuces by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2004, 10(5): 3-4
Authors:LI Cheng-wang
Affiliation:Anhui Institute of Medical Science, Hefei 230061, China
Abstract:
Objectives: To determine the content of chlorogenic acid in Yinqiaojiedu Granuces by HPLC. Method: The analysis was performed on an hypersil C18 column, with a mobile phase of methanol-water-acetic acid-glacial-triethylamine (15:85:1:0.2), the detection wavelength was 254nm. Result: Recovery of chlorogenic acid was 98.12% RSD=0.97%(n=5). Conclusion:This method is simple and reproducible, and it can be used for the quantitative analysis of chlorogenic acid in Yinqiaojiedu Granuces.
Keywords:Chlorogenic acid  Yinqiaojiedu Granuces  HPLC
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