基于生物热力学表达的麻黄汤和麻杏石甘汤的寒热药性比较

目的：从生物热力学的角度,探讨麻黄汤与麻杏石甘汤的药性差异的客观真实性。方法：采用微量量热学方法,分别测定在中药麻黄汤与麻杏石甘汤不同浓度水煎液作用下,金黄色葡萄球菌生长代谢的热谱图(即热功率-时间曲线,P-t图),并根据热动力学模型lnP_t=lnP₀+kt,计算得到一系列生物热动力学参数。结合中药药性理论,综合分析麻黄汤与麻杏石甘汤的药性差异。结果：麻黄汤与麻杏石甘汤均能不同程度地抑制金黄色葡萄球菌的生长,且随着药液浓度的增加,其抑菌作用逐渐增强。但温热药方麻黄汤较寒凉药方麻杏石甘汤对金黄色葡萄球菌的抑制作用弱,同时,从热动力学参数中的最大热输出功率计算结果也表明,麻黄汤作用于细菌生长代谢时,所释放的热量要高于麻杏石甘汤,两者存在较稳定的差异。结论：应用生物热力学方法进一步探索和验证了麻黄汤和麻杏石甘汤的药性差异。

Comparison of properties of Mahuang decoction and Maxing Shigan decoction based on biothermodynamics

Objective: To probe into the objectivity and authenticity of four properties beteen Mahuang decoction and Maxing Shigan decoction from biothermodynamics. Method: The power-time curves of growth of Staphylococcus aureus at different concentrations between Mahuang decoction and Maxing Shigan decoction were determined by TAM Air Isothermal Calorimeter. The growth rate constants of promotive and inhibitory actions were calculated. Moreover, the difference of properties beteen Mahuang decoction and Maxing Shigan decoction was analyzed from the point of view of TCM theory. Result: Both the Mahuang decoction and Maxing Shigan decoction could inhibit the growth and metabolism of Staphylococcus aureus. The k and P_m were decreased with the mass increase of the decoction. However, inhibitory activity of Mahuang decoction with warm property was weaker than that of Maxing Shigan decoction with cool property. Moreover, Mahuang decoction decreased heat output in growth metabolism more weakly than Maxing Shigan decoction. There was a stable difference between them. Conclusion: Studying on biothermodynamics can show the difference of four properties of Traditional Chinese Medicine. So, it provides a new and useful means for the study of the properties of traditional Chinese medicine.