Hedgehog信号通路在人肝癌细胞株中的表达及意义

目的 探讨Hedgehog信号通路在人肝细胞癌细胞系中的表达及意义。 方法 采用半定量 RT-PCR 法检测PLC/PRF/5、HepG2及 SMMC-7721肝癌细胞株中Shh、Ptch1、Smo、Gli1、Gli2 mRNA 的表达；Western blot法检测PLC/PRF/5、HepG2及 SMMC-7721肝癌细胞株中Gli2蛋白的表达。 结果 除Shh外， Ptch1、Smo、Gli1、Gli2 mRNA在PLC/PRF/5、HepG2及 SMMC-7721细胞中均有不同程度表达。其中，Ptch1、Smo、Gli1、Gli2 mRNA在PLC/PRF/5及SMMC-7721细胞中的表达强度显著高于成人正常肝细胞，而Gli1 mRNA在HepG2细胞中的表达量与正常肝细胞无明显差异；Gli2蛋白在成人正常肝细胞中几乎没有表达，在HepG2细胞中的表达也与正常肝细胞无明显差别，而在PLC/PRF/5及 SMMC-7721细胞中，尤其是SMMC-7721细胞，Gli2的表达量异常增高。 结论 Hedgehog 信号通路的异常激活参与了肝细胞癌的发生发展过程，Gli2可能成为肝细胞癌重要生物学标志和生物治疗靶点。

Expression and significance of Hedgehog signaling pathway in human hepatocellular carcinoma cell strains

Objective To investigate the expression and significance of Hedgehog signaling pathway in human hepatocellular carcinoma cell strains.Methods Semi-quantitative RT-PCR was used for detections of Shh,.Ptch1,.Smo,.Gli1 and Gli2 mRNA,.and Western blot was used for detections of Gli2 protein in PLC/PRF/5、.HepG2 and SMMC-7721 hepatocellular carcinoma cell strains.Results Besides Shh,mRNA of Ptch1,.Smo,.Gli1,.Gli2 had expressions of different degree in PLC/PRF/5,HepG2 and SMMC-7721 cells.The mRNA expressions of Ptch1,Smo,Gli1,and Gli2 were significantly higher in PLC/PRF/5 and SMMC-7721 than that in normal adult liver cells,while mRNA expressions of Gli1 was no significant differences between HepG2 cells and adult liver cells.Gli2 protein was almost undetected in adult liver cells,.and was no significant differences between HepG2 cells and adult liver cells,.but Gli2 protein was highly expressed in PLC/PRF/5 and SMMC-7721 cell,particularly in SMMC-7721.Conclusion Abnormal activation of Hedgehog pathway may take part in development and progression of human HCC,.Gli2 may be an important biological marker of HCC and biological therapeutic target.