失血性休克大鼠输注6%羟乙基淀粉对网状内皮系统功能的影响

目的 观察6％羟乙基淀粉（HES 200／0．5）用于纠正大鼠失血性休克对网状内皮系统功能的影响。方法 30只Wistar大鼠随机分为HES组和对照组（低分子右旋糖酐组）各15只。放血至平均动脉压5.33kPa(1kPa=7.5mmHg)，并用回输或放血方法维持该血压60min，复制失血性休克模型。输入与出血量相同体积的HES或低分子右旋糖酐，分别在复苏后12h(T1)、24h(T2)、48h（T3）、72h(T4)4个时间点，每个时间点分离、纯化3只大鼠肝脏枯否氏细胞（KC）计数KC吞噬Latex 乳胶颗粒的数量。结果 在同一时间点，HES组与低分子右旋糖酐KC吞噬功能无显著性差异；两组输液后任一时间点与T0（未经过休克及复苏，且未输注任一液体）点KC吞噬功能相比无显著性差异。结论 在机体失血量达血容量的20％－30％时输注相同体积的羟乙基淀粉溶液，对肝脏KC吞噬功能无显著影响。

The effect of hydroxyethyl starch (6 %, 200/0.5) on reticuloendothelial system function in hemorrhagic shock in rat

Objective To examine the effect of hydroxyethyl starch (HES 6%, 200/0.5) on reticuloendothelial system (RES) function in hemorrhagic shock. Methods Thirty 8 to 12-week-old Wistar rats weighing 250-350g were randomly divided into two groups: HES group ( n = 15) and control group ( n =15) . The animals were anesthetized with intraperitoneal 0.5% pentobarbital sodium 30mg ?kg-1 Bilateral common carotid arteries were exposed and cannulated for MAP monitoring and blood-letting. Blood was gradually removed until MAP dropped to 40mm Hg, which was maintained for 60min. Then same amount of HES (HES group) or Dextran (control group) was infused. The Kupffer cells were separated and purified before hemorrhagic shock (T0), at 12h (T1) 24h (T2) 48h (T3) and 72h (T4) after HES or DEX infusion, when the animals recovered from shock. Phagocytic activity of Kupffer cells (KC) was measured by counting the amount of Latex particles ingested by KC. Results At the same intervals (T1-4) there was no significant difference in phagocytic activity of KC between the two groups. There was no significant difference in phagocytic activity of KC between T0 (baseline) and T1-4 in both groups.Conclusions The phagocytic activity of Kupffer cells in not affected by removed of 20-30% blood volume which was replaced by same amount of HES.