维生素C对CD4+效应记忆性T细胞体外扩增的影响

 目的：探讨小分子药物维生素C（VC）对CD4+效应记忆性T细胞（TEM）体外扩增的影响从而改善过继免疫治疗的效果。方法：体外分离正常人的外周血CD4+ T淋巴细胞,将细胞分为2组（实验组和对照组）进行细胞培养。实验组加入VC后,通过细胞计数仪和流式细胞仪对2组细胞中TEM的扩增情况进行检测。结果：(1) 在CD4+ T细胞体外扩增实验中,VC对CD4+ T细胞的总数无显著影响。(2) VC使TEM在CD4+ T细胞中的比例显著升高,100 mg/L为扩增的最佳浓度。(3) 在CD4+ T细胞扩增的第10天检测其中TEM的细胞数量,对照组TEM的数量为(1.22±0.15)×106,实验组TEM的数量为(3.56±0.35)×106 ,两者的差异有统计学意义（P<0.01）。结论: VC可有效地促进CD4+ TEM的体外扩增,为过继免疫治疗提供简单、安全及有效的体外扩增方法。

Effect of vitamin C on expansion of CD4 + effector memory T cells in vitro

AIM：To investigate the effect of vitamin C (VC), a small-molecule compound, on the expansion of CD4+ effector memory T cells (TEM) in vitro and to improve the effect of adoptive immunotherapy. METHODS：Normal human peripheral blood CD4+ T-lymphocytes were isolated and randomly divided into 2 groups: control group and experiment group. VC was added into experimental group. Thereafter, the expansion of CD4+ TEM in the 2 groups was detected by cell counter and flow cytometry. RESULTS：VC did not significantly affect the total number of CD4+ T cells, while it raised the ratio of TEM in CD4+ T cells at an optimal concentration of 100 mg/L. After 10 days of the expansion of CD4+ T cells, the number of CD4+ TEM in experiment group was (3.56±0.35)×106, significantly larger than that in control group ［(1.22±0.15)×106, P<0.01］. CONCLUSION: VC can effectively promote the expansion of CD4+ TEM in vitro, which provides a simple, safe and effective expansion method for adoptive immunotherapy.