| 钴原卟啉诱导HO-1高表达抗大鼠心肌缺血/再灌注损伤的实验研究 |
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| 引用本文： | 陆卫华,曾尟枚,邵素花,唐忠志,陈忠庆.钴原卟啉诱导HO-1高表达抗大鼠心肌缺血/再灌注损伤的实验研究[J].重庆医学,2009,38(11). |
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| 作者姓名： | 陆卫华 曾尟枚 邵素花 唐忠志 陈忠庆 |
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| 作者单位： | 广州军区武汉总医院急诊科,湖北,430070 |
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| 摘 要： | 目的 探讨血红素氧合酶-1(HO-1)对大鼠心肌缺血/再灌注损伤的保护效应及作用机制.方法 采用HO-1诱导剂钴原卟啉(CoPP) 和抑制剂锌原卟啉(ZnPP) 分别进行干预处理后建立大鼠心肌缺血/再灌注损伤模型,观察大鼠再灌注后I/R左心室心肌超微结构变化;检测HO-1蛋白在大鼠心肌的表达情况;测定大鼠左心室心肌组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量.结果 再灌注前使用CoPP 进行预处理可以诱导HO-1蛋白表达上调.HO-1蛋白表达上调可以减少缺血/再灌注后心肌细胞坏死,提高心肌组织中SOD含量,并降低MDA含量.结论 CoPP诱导的HO-1过表达可以抑制心肌缺血/再灌注损伤后细胞坏死,从而减轻心肌再灌注损伤,抗氧自由基是其机制之一.
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| 关 键 词： | 血红素氧合酶-1 心肌缺血/再灌注损伤 保护作用 抗氧自由基 |
Protective effect of HO-1 on myocardial ischemia/reperfusion injury in rats |
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| LU Wei-hua,ZENG Xian-mei,SHAO Su-hua,et al..Protective effect of HO-1 on myocardial ischemia/reperfusion injury in rats[J].Chongqing Medical Journal,2009,38(11). |
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| Authors: | LU Wei-hua ZENG Xian-mei SHAO Su-hua |
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| Institution: | LU Wei-hua,ZENG Xian-mei,SHAO Su-hua,et al.(Department of Emergency,Wuhan General Hospital of PLA Guangzhou Military Comm,,Wuhan 430070,China) |
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| Abstract: | Objective To investigate the protective effects of HO-1 on myocardial ischemia/reperfusion injury of rats in vivo,and explore the possible mechanism.Methods Rat myocardial ischemia/reperfusion injury model was established.The expression of HO-1 protein was detected,the vitality of SOD and the quantity of MDA was detected in rat myocardial ischemia/reperfusion injury model treated with Cobalt protoporphyrin(HO-1 inducer) or Zinc protoporphyrin(HO-1 inhibitor).Results Pretreatment of CoPP before reperfusion c... |
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| Keywords: | heme oxygenase-1 myocardial ischemic reperfusion injury protective effects anti-oxygen free radicals |
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