牛心包脱细胞基质的研制

目的为组织工程学方法研制生物瓣提供适合的基质材料。方法用去污剂-酶联合四步法脱除牛心包组织中的细胞,并对处理后组织的理化性质进行分析、测试。结果脱细胞效果良好,且能较好地保持胶原纤维和弹性纤维的排列分布。脱细胞后的牛心包组织的厚度、抗拉负荷、抗拉强度、断裂伸长率和热皱缩温度无明显变化。组织中可溶性蛋白含量无显著变化,而作为结构成分的胶原蛋白和蛋白聚糖得到了较好的保存。结论去污剂-酶联合四步法能有效地制造牛心包脱细胞基质材料。

A study on cellular matrix via cell extraction from bovine pericardium

OBJECTIVE: To obtain an acellular matrix from bovine pericardial tissue, as a scaffold for tissue engineering of heart valves or cardiovascular patching applications. METHODS: A four step detergent and enzyme link extraction procedure was practiced in this study. The mechanic properties of the acellular matrix were evaluated, and the components were analyzed biochemically. RESULTS: HE staining confirmed the removal of cells and Von Gieson staining showed the integrality of collagen and elastin. Biochemical analysis demonstrated the retention of collagen and some glycosaminoglycans while the percentage of the soluble proteins reduced slightly. The tissue fracture strength and shrinkage temperature made no significant difference after extraction. CONCLUSIONS: The extraction process is effective to remove cells from bovine pericardial tissue while maintains its mechanical strength. This approach may eventually lead to a scaffold for heart valves and cardiovascular patching applications in tissue engineering.