白细胞介素-12p403'非翻译区基因多态性与丙型肝炎病毒感染的相关性

目的 探讨丙型肝炎患者IL-12p40 3'非翻译区rs3212227位点基因多态性与HCV感染的关系.方法 应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)和DNA测序的方法榆测127例丙型肝炎患者IL-12 p40 3'非翻译区rs3212227位点的基因型,采用荧光定量PCR技术测定丙型肝炎患者血清HCV RNA水平.组间基因型及等位基因分布频率比较采用χ2检验.结果 丙型肝炎患者IL-12 p40 3'非翻译区rs3212227位点AA、AC、CC基因型分布频率分别为34.6%、40.9%和24.4%.等位基因A、C分布频率分别为55.1%、44.9%.丙型肝炎患者中HCVRNA≥2.0×106拷贝/mL组IL-12 p40 rs3212227位点C等位基因携带者分布频率明显高于HCVRNA<2.0×106拷贝/mL组(χ2=7.367,P=0.007).IFN无应答组IL-12 p40 rs3212227位点C等位基因分布频率明显高于IFN应答组(χ2=4.942,P=0.026).结论 IL-12p40 3'非翻译区rs3212227位点基因多态性与HCV感染具有相关性,携带C等位基因的个体可能更利于HCV复制,而不利于IFN治疗.

Abstract：

Objective To investigate the association between single nucleotide polymorphism (SNP) of interleukin-12 (IL-12) p40 3'untranslated region rs3212227 site and hepatitis C virus (HCV) infection. Methods Patients with hepatitis C (n=127) were genotyped and analyzed for the SNP of IL-12 p40 rs3212227 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. The serum HCV RNA levels of patients with hepatitis C were detected using real time fluorescence quantitative-polymerase chain reaction (FQPCR). Inter-group comparisons of genotype and allele frequency were analyzed using chi-square test.Results In patients with hepatitis C, the frequencies of AA, AC and CC genotypes of IL-12 p40 rs3212227 site were 34. 6% ,40. 9% and 24. 4% , respectively,and the frequencies of allele A, C of IL12 p40 rs3212227 site were 55.1% and 44. 9%, respectively. The frequency of rs3212227 C allele in patients with HCV RNA ≥2. 0× 106 copy/mL was higher than that in patients with HCV RNA <2. 0 ×106 copy/mL (χ2 =7. 367, P = 0. 007). The frequency of rs3212227 C allele in responders to interferon (IFN) therapy was lower than that in patients with nonresponse to IFN therapy (χ2 =4. 942,P=0. 026). Conclusions The SNP of rs3212227 is correlated with HCV infection. The carriers with C allele may be susceptible to HCV infection, while resistant to IFN therapy.

Association between genetic polymorphisms of interleukin-12 p40 3' untranslated region and hepatitis C virus infection

Objective To investigate the association between single nucleotide polymorphism (SNP) of interleukin-12 (IL-12) p40 3'untranslated region rs3212227 site and hepatitis C virus (HCV) infection. Methods Patients with hepatitis C (n=127) were genotyped and analyzed for the SNP of IL-12 p40 rs3212227 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. The serum HCV RNA levels of patients with hepatitis C were detected using real time fluorescence quantitative-polymerase chain reaction (FQPCR). Inter-group comparisons of genotype and allele frequency were analyzed using chi-square test.Results In patients with hepatitis C, the frequencies of AA, AC and CC genotypes of IL-12 p40 rs3212227 site were 34. 6% ,40. 9% and 24. 4% , respectively,and the frequencies of allele A, C of IL12 p40 rs3212227 site were 55.1% and 44. 9%, respectively. The frequency of rs3212227 C allele in patients with HCV RNA ≥2. 0× 106 copy/mL was higher than that in patients with HCV RNA <2. 0 ×106 copy/mL (χ2 =7. 367, P = 0. 007). The frequency of rs3212227 C allele in responders to interferon (IFN) therapy was lower than that in patients with nonresponse to IFN therapy (χ2 =4. 942,P=0. 026). Conclusions The SNP of rs3212227 is correlated with HCV infection. The carriers with C allele may be susceptible to HCV infection, while resistant to IFN therapy.