复方扶芳藤合剂含药血清对大鼠骨髓间充质干细胞Notch信号通路的影响

目的:观察复方扶芳藤合剂含药血清对大鼠骨髓间充质干细胞(BMSCs) Notch信号通路的影响.方法:采用全骨髓贴壁法分离纯化培养大鼠骨髓间充质干细胞；制备复方扶芳藤合剂含药血清,将30只SD大鼠随机分为2组,每组随机15只,分别为ig复方扶芳藤合剂2 g·kg-1 ·d-1 ig的药物干预组和生理盐水2 mL·kg-1·d-1ig的阴性对照组.取10％的含药血清及阴性对照组血清分别干预大鼠BMSCs,采用RT-PCR方法检测Notch1,Jagged1,Hes1 mRNA表达变化；采用免疫细胞化学法检测Notch1,Jagged1表达变化.结果:RT-PCR显示药物干预组Notch1,Jagged1,Hes1的mRNA表达水平均高于阴性对照组(P＜0.05),免疫细胞化学显示药物干预组Notch1,Jagged1蛋白表达均高于阴性对照组(P＜0.05),差异有统计学意义.结论:复方扶芳藤合剂含药血清可以引起大鼠BMSCs Notch信号通路上的关键基因和蛋白表达上调,激活Notch信号通路.

Effect of Compound Fufangteng Mixture on Bone Marrow Hematopoietic Stem Cells Notch Signal Pathway in Rats

Objective: To observe the effect of compound Fufangteng mixture medicated serum on bone marrow hematopoietic stem cells (BMSCs) Notch signal pathway in rats. Method: The whole bone marrow adherent method was used to isolate, culture and purify the BMSCs in vitro.A total of 30 rats was randomly divided into 2 groups, 15 each group, and compound Fufangteng mixture 2 g·kg^-1·d^-1 was given by gavage to the rats as drug intervention group, while physiological saline 2 mL·kg^-1·d^-1 was given as negative control group. The 10% medicine serum and negative control serum were intervene to BMSCs. Then RT-PCR was used to detect the mRNA expression of Notch1,Jagged1 and Hes1 after intervention. And the immunofluorescence cytochemical method was used to detect the protein expression of Notch1 and Jagged1 after the intervention. Result: RT-PCR results showed that the mRNA expression of Notch1,Jagged1 and Hes1 in drug intervention group was higher than negative control group(P<0.05). Immunofluorescence cytochemical method indicated that the protein expression of Notch1 and Jagged1 in drug intervention group was higher than that in negative control group(P<0.05). Conclusion: Compound Fufangteng mixture containing serum can upregulate key genes of Notch signal pathway and protein expression, and activate Notch signal pathway.