| 聚乙二醇沉淀联合电化学发光法检测巨泌乳素血症实验条件优化 |
| |
| 引用本文： | 丁杰锋,张荣富,屠凤娟,夏舟岚,丁丁.聚乙二醇沉淀联合电化学发光法检测巨泌乳素血症实验条件优化[J].医学研究杂志,2008,37(3):46-48. |
| |
| 作者姓名： | 丁杰锋 张荣富 屠凤娟 夏舟岚 丁丁 |
| |
| 作者单位： | 1. 浙江省绍兴市妇女儿童医院,312000
2. 浙江大学药学院,杭州,310058 |
| |
| 基金项目： | 浙江省绍兴市科技局重点科研项目 |
| |
| 摘 要： | 目的 通过PEG沉淀预处理的实验条件(沉淀时温度,相对离心力和离子强度)优化,使PEG沉淀预处理联合电化学发光检测筛检结果与凝胶层析法检测巨泌乳素血症结果具有可比性.方法 通过同定离心时间,优化离心速度、温度及离子强度,达到优化实验条件的目的.结果 25%PEG 6000 1M PBS沉淀液,选择离心速度为1800g,离心温度18℃,离心时间为10min,其处理后的结果最接近凝胶层析法合并电化学发光法测得小分子PRL浓度.结论 优化后的聚乙二醇沉淀联合电化学发光法适合用于巨分子泌乳素血症的筛检.
|
| 关 键 词： | 样品预处理 巨泌乳素血症 条件优化 聚乙二醇 沉淀 化学发光法 发光检测 泌乳 素血症 实验条件优化 Detect Test Electrochemiluminescence Associated Precipitation Experimental 浓度 分子 预处理 离心速度 选择 温度及 离心时间 |
| 修稿时间： | 2007年10月24 |
Optimization of Experimental Conditon in the PEG Precipitation Associated with Electrochemiluminescence Test to Detect Macroprolactinaemia |
| |
| Ding Jiefeng,Zhang Rongfu,et al..Optimization of Experimental Conditon in the PEG Precipitation Associated with Electrochemiluminescence Test to Detect Macroprolactinaemia[J].Journal of Medical Research,2008,37(3):46-48. |
| |
| Authors: | Ding Jiefeng Zhang Rongfu |
| |
| Institution: | Ding Jiefeng,Zhang Rongfu,et al.Shaoxing Women , Child Health Hospital,Zhejiang 312000,China |
| |
| Abstract: | Objective The experimental condition of PEG precipitation pre-treatment method associated with electrochemiluminescence was optimized to gain a coincident result compared with gel chromatography method.Methods A fixed centrifuge time,optimized centrifuge speed,temperature and iron intensity were used to achieve a best experimental condition.Results The detection concentration of micromolecular PRL was approximate to gel chromatography method under the experimental condition of being centrifuged 10 minutes a... |
| |
| Keywords: | Pre-treatment method Macroprolactinemia Optimization of conditon |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《医学研究杂志》浏览原始摘要信息 |
| 点击此处可从《医学研究杂志》下载免费的PDF全文 |
