家族性肌萎缩侧索硬化动物模型脊髓侧角神经元特征

目的探讨脊髓侧角神经元在SOD1G93A转基因鼠发病过程中的变化特征。方法利用甲苯胺蓝染色及免疫组化的方法观察SOD1G93A转基因小鼠不同病变时期上、中、下胸髓侧角神经元的数量变化特征。结果甲苯胺蓝染色法定量研究发现不同时期SOD1G93A转基因小鼠上胸髓、下胸髓水平,症状前期和发病期神经元计数较终末期均有显著性差别(P<0.05);与对照组相比,终末期胸髓各段侧角神经元计数明显减少且有统计学意义;免疫组化发现SOD1G93A转基因小鼠上胸髓、中胸髓水平不同时期相比,症状前期和发病期乙酰胆碱酯酶(AchE)阳性神经元计数较终末期均有显著性差别(P<0.05);与对照组相比,发病期上胸髓及终末期中、下胸髓水平侧角AchE阳性神经元数量减少具有统计学意义;结论在SOD1G93A转基因鼠发病过程中,存在脊髓侧角神经元丢失,尤其是在疾病的终末期,上、中、下胸髓节段间无明显差异。

The characteristic of neurons in lateral horn of spinal cord of family amyotrophic lateral sclerosis animal model

Objective To discuss the feature of lateral horn of spinal cord in ALS mice at different stage.Methods All were tested with Nissl staining and immunohistochemical to count the neurons in the lateral horn of the spinal cord.Results Quantitative study of toluidine blue stain found that at the upper and lower thoracic level of the spinal cord the cell counts were apparently different(P<0.05) at both presymptomatic and diease onset stage compared with end stage.Compared with the control group,the intermediolateral column(IML) cell counts decreased significantly(P<0.05) at the diease onset stage of upper level and end-stage of all levels of the spinal cord;the neurons in the presymptomatic stage and disease onset stage are nomal,but those at the end stage were degeneratied.Immunohistochemical study found that in the upper and middle level of the spinal cord,at both presymptomatic and diease onset stage the cell counts of acetylcholinesterase(AchE) Positive neurons were apparently different(P<0.05) compared with end stage.compared with the control group,the IML cell counts decreased significantly(P<0.05) at the diease onset stage of upper level and end-stage of all levels of the spinal cord.Conclusion The degeneration and loss of the intermediaolateral nucleus of spinal cord exist in ALS,especially at the end stage of the disease,in additon,it has no apparent difference between upper,middle and lower level of the thoracic spinal cord.