32P-Postlabelling method for the detection of 7-alkylguanine adducts formed by the reaction of different 1,2-alkyl epoxides with DNA |
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| Authors: | Kumar, Rajiv Staffas, Joakim Forsti, Asta Hemminki, Kari |
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| Affiliation: | Center for Nutrition and Toxicology, Karolinska Institute NOVUM, 141 57, Huddinge, Sweden |
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| Abstract: | ![]()
A 32P-postlabelling method is reported for the detection of7-alkylguanines, the major adducts formed by the reaction of1,2-alkylepoxides with DNA. Calf thymus DNA was reacted in vitrowith different epoxides (ethylene oxide through octylene oxide)and digested with micrococcal nuclease and spleen phosphodiesteraseto 3'-nucleotides. The adduct enrichment was carried out byan ion-exchange method and adducts were labelled with [ |
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