乙肝患者HBV-LP与HBV-DNA表达的相关性及临床应用评价

目的 探讨不同模式乙型肝炎患者中乙肝病毒大蛋白（HBV-LP）与乙肝病毒标志物（HBV M）、乙肝病毒前S1抗原（HBV perS1）及HBV-DNA表达的相关性,评价其在乙肝诊断和治疗中的临床应用价值.方法 采用酶联免疫吸附试验（ELISA）法,对286例HBV感染者和45名健康对照者血清分别检测HBV-LP、HBV前S1抗原、乙型肝炎病毒五项标志物的表达水平;采用实时荧光定量PCR法检测HBV-DNA拷贝数;分析HBV-LP与其它方法的相关性.结果 在286例HBV感染者血清HBV-LP阳性237例,阳性率82.9%.其中乙肝大三阳HBV-DNA阳性92例,阳性率82.8%,HBV-LP阳性94例,阳性率84.7%,两者无显著性差异（P〉0.05）,阳性符合率为79.6%;HBVperS1阳性185例,阳性率64.7%,两者有差异性显著（P〈0.05）;HBV DNA拷贝数与HBV-LP阳性间呈正相关.结论 血清HBV-LP作为HBV患者临床诊断的一项新指标,较准确地反映了乙肝病毒的复制情况,是HBV-M有益的补充,在HBV病毒复制、疗效及预后监测中具有重要的意义.

Evaluation on the correlation between HBV-DNA and serum HBV-LP of patients with hepatitis B and its clinical application.

Objective To investigate the correlation between serum protein HBV - LP of patients with hepatitis B virus （HBV） infection and HBV - DNA. The significance in the diagnosis and treatment of hepatitis B were also investigated. Methods Enzyme - linked immunosorbent assay （ELISA） were used to detect serum level of HBV - LP , HBV preS1 antigen and hepatitis B markers （ HBsAg, HBsAb, HBeAg, HBeAb, HB- cAb）. Real - time fluorescence quantitative PCR were applied to detect HB V - DNA copy number for 286 cases with HBV infection and 45 cases of health control. Correlation between HBV - LP and other methods were statistically analyzed. Results Serum HBV - LP was positive in 237 cases of 286 HBV patients. The positive rate was 82.9%. HBV - DNA of HB - sAg, HBeAg and HBcAb of hepatitis B was positive in 92 cases and the posi- tive rate was 82.8%. HBV - LP was positive in 94 cases and the positive rate was 84.7%. There was no significant difference （ P 〉 0.05 ）, and the positive coincidence rate was 79.6%. HBV preS1 antigen was positive in 185 cases and the positive rate was 64.7%. There was significant difference （ P 〈 0.05 ）. HBV DNA copy number was positively correlated with HBV - LP. Conclusion As a new indicator for clinical diagnosis of HBV pa- tients, serum HBV - LP reflects HBV replication more accurately, and it is a useful complement for HBV - M. It has important significance in HBinfection monitoring, viral replication, disease progression, antiviral efficacy, liver cell injury and prognosis observation.