α-转化生长因子及其受体在人肝脏组织硬化过程中的表达特征

背景:α-转化生长因子及表皮生长因子受体在不同病变中的表达和意义不尽相同。目的:观察α-转化生长因子及表皮生长因子受体在人类肝硬变组织中的表达。设计:非随机对照实验。材料:实验于2003-03/2004-05在解放军第四军医大学西京医院病理科完成。63份人类肝硬变组织选自解放军第四军医大学西京医院外科手术标本(患者知情同意),5份正常人类肝脏组织取自第四军医大学病理学教研室尸检组织(排除有肝脏疾病)。方法:运用免疫组织化学和原位杂交的方法对63份人类肝硬变组织及5份正常人类肝脏组织进行研究,计算α-转化生长因子及表皮生长因子受体阳性率并进行统计学处理。主要观察指标:免疫组织化学染色方法和原位杂交染色检测肝硬变组织中α-转化生长因子及表皮生长因子受体的表达。结果:免疫组织化学方法检测α-转化生长因子与表皮生长因子受体在63份肝硬变组织中的阳性率分别为84%(53/63)及52%(33/63),阳性产物为棕黄色颗粒,主要弥漫分布于肝细胞浆内,α-转化生长因子与表皮生长因子受体的表达在肝硬变组织中呈显著正相关(r=0.32,P<0.05),5份正常肝组织中α-转化生长因子与表皮生长因子受体表达为阴性;α-转化生长因子与表皮生长因子受体的原位杂交阳性率(86%,54%)略高于其免疫组织化学结果,两种检测方法之间差异无显著性(P>0.05)。结论:在肝脏组织硬变形成过程中可能存在α-转化生长因子/表皮生长因子受体自分泌循环,而α-转化生长因子、表皮生长因子受体高水平表达是促使肝脏组织硬变形成的重要因素之一。

Expression of transforming growth factor-alpha and its receptor during the process of human liver cirrhosis

BACKGROUND: The expression and significance of transforming growth factor-alpha (TGF-α) and epidermal growth factor receptor (EGF-R) in different diseases are different.OBJ ECTTVE: To investigate the expression of TGF-α and EGF-R in human liver cirrhosis tissues.DESIGN : Non-randomized controlled trial.SETTING: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University of Chinese PLA.MATERIALS: This trial was carried out in the Department of Pathology, Xijing Hospital, Fourth Military Medical University of Chinese PLA during March 2003 to May 2004. Sixty-three samples of human liver cirrhosis tissue were obtained from Department of General Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA (Informed consents were obtained). Five human normal liver tissues were obtained form autopsy in Department of Pathology, Fourth Military Medical University of Chinese PLA (patients with liver diseases were excluded).METHODS: Sixty-three samples of human liver cirrhosis specimens and five human normal liver tissues were studied with immunohistochemical method and in situ hybridization technique. The positive rates of TGF-α and EGF-R were calculated and managed statistically.MAIN OUTCOME MEASURE: Detection of the expressions of TGF-α and EGF-R in liver cirrhosis tissues were detected with immunohistochemical method and in situ hybridization technique.RESULTS: The expression rate of TGF-α and EGF-R in 63 samples of human liver cirrhosis tissues was 84% (53/63)and 52% (33/63) respectively. These positive granules were brown and most of them were in cytoplasm of hepatocytes.There was significantly positive correlation between TGF-α and EGF-R (r =0.32,P ＜ 0.05). In five normal liver tissues,TGF-α and EGF-R were not detectable in hepatocytes and bile ducts. In situ hybridization showed the levels of TGF-αmRNA and EGF-R mRNA were higher (86%, 54%) than those of immunohistochemistry. There was no significant difference between two detective methods (P＞0.05).CONCLUSrON: Liver cirrhosis might be under the autocrine regulation of TGF-α/EGF-R. Increasing expression of TGF-αand EGF-R might be one of important factors in liver cirrhosis pathogenesis.