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| RNA干扰ERCC1基因表达对卵巢癌顺铂敏感性的影响 | |
| 引用本文： | 刘静,糜若然,瞿全新,熊冬生. RNA干扰ERCC1基因表达对卵巢癌顺铂敏感性的影响[J]. 中国现代医学杂志, 2008, 18(10): 1338-1341 |
| 作者姓名： | 刘静 糜若然 瞿全新 熊冬生 |
| 作者单位： | 1. 天津市中心妇产科医院,妇科,天津,300052 2. 天津医科大学总医院,妇产科,天津,300052 3. 中国医学科学院血液学研究所,药物室,天津,300052 |
| 摘 要： | 目的研究ERCC1基因表达与卵巢癌顺铂耐药的关系。构建携带ERCC1基因小发夹干扰RNA的重组质粒表达载体,观察RNA干扰ERCC1基因表达对卵巢癌顺铂敏感性的影响。方法RT-PCR方法检测COC1、COC1/DDP细胞中ERCC1基因表达,基因重组技术构建携带ERCC1小发夹干扰RNA的重组质粒表达载体,脂质体法转染COC1/DDP细胞,RT-PCR及westernblot方法检测转染后细胞内ERCC1mRNA和蛋白的表达,MTT法检测转染后细胞对顺铂敏感性的改变。结果COC1/DDP细胞中ERCC1基因表达明显高于COC1细胞。重组质粒转染后,COC1/DDP细胞中ERCC1mRNA和蛋白表达显著下调。转染后细胞对顺铂的敏感性显著增加。结论COC1/DDP中ERCC1基因表达增强与卵巢癌顺铂耐药相关。RNA干扰抑制ERCC1基因表达能增加卵巢癌耐药细胞COC1/DDP对顺铂的敏感性。 |
| 关 键 词： | RNA干扰 ERCC1基因 卵巢癌 顺铂 干扰抑制 基因表达 卵巢癌 顺铂敏感性 影响 cell lines ovarian cancer cisplatin sensitivity gene repair excision expression interfering 耐药细胞 相关 增强 蛋白表达 mRNA 质粒转染 |
| 文章编号： | 1005-8982(2008)10-1338-04 |
| 修稿时间： | 2008-01-12 |
Effect of RNA interfering expression of excision repair cross-complementing 1 gene on sensitivity to cisplatin in ovarian cancer cell lines |
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| LIU Jing,MI Ruo-ran,QU Quan-xin,XIONG Dong-sheng. Effect of RNA interfering expression of excision repair cross-complementing 1 gene on sensitivity to cisplatin in ovarian cancer cell lines[J]. China Journal of Modern Medicine, 2008, 18(10): 1338-1341 | |
| Authors: | LIU Jing MI Ruo-ran QU Quan-xin XIONG Dong-sheng |
| Affiliation: | LIU Jing1,MI Ruo-ran2,QU Quan-xin2,XIONG Dong-sheng3 (1.Department of Gynecology,Tianjin Central Hospital of Obstetrics , Gynecology,Tianjin 300052,P.R.China,2.Department of Obstetric , Gynecology,General Hospital of Tianjin Medical University,3.Department of Pharmacy,Institute of Hematology,Chinese Academy of Medical Sciences,P.R.China) |
| Abstract: | [Objective] To study the relationship between Cisplatin drug-resistance and the expression of ERCC1 gene in ovarian cancer cell lines. Recombinant plasmid carrying ERCC1-shRNA was constructed and the influence of RNA interfering the expression of ERCC1 on the sensitivity of ovarian cancer to Cisplatin was investigated.[Methods] RT-PCR was used to detect the expression of ERCC1 mRNA in COC1 and COC1/DDP cells. The recombinant plasmid vector carrying small hairpin interfering RNA targeting ERCC1 gene was cons... |
| Keywords: | RNA interference ERCC1 gene ovarian cancer Cisplatin |
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