胆囊收缩素酶联免疫测定法的研究

为探索多肽类物质的酶联免疫测定方法，采用紫外线照射及戊二醛活化酶标板包被八肽胆囊收缩素（ＣＣＫ８），并以此为基础进行酶联免疫吸附试验。结果显示：该方法可使ＣＣＫ８稳固地结合于酶标板上；竞争性抑制实验中游离的ＣＣＫ８与固相ＣＣＫ８可与抗体发生竞争结合，并呈良好的定量关系。该方法简便、重复性及稳定性好（批内及批间变异系数分别为４７５％及７８０％），可用于ＣＣＫ相关肽初步的结构分析。

Study on Enzyme Linked Immunosorbent Assay for Cholecystokinin

This study was undertaken to develop a method for assaying octapeptide cholecystokinin(CCK 8) using ELISA.The design of the method was based on the pretreatment of polystyrene microplates with ultraviolet irradiation and glutaraldehyde activation piror to coating.The results showed that this pretreatment let to stable attachment of CCK 8 to the solid phase.Competitive ELISA with CCK 8 as a competitor gave excellent quantitive relationship.Using this method we distinguished two kinds of CCK related peptides.This method proved to be simple,stable and reproducible;the intra and inter assay coefficients of variation were 4 75% and 7 80% respectively.