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| 双着丝粒染色体自动分析生物剂量估算影响因素研究 | |
| 引用本文： | 冯骏超,张琳,戴宏,刘玉龙,卞华慧,陈炜博,王优优,马娅,刘伟. 双着丝粒染色体自动分析生物剂量估算影响因素研究[J]. 中国辐射卫生, 2018, 27(4): 289-293. DOI: 10.13491/j.issn.1004-714x.2018.04.001 |
| 作者姓名： | 冯骏超 张琳 戴宏 刘玉龙 卞华慧 陈炜博 王优优 马娅 刘伟 |
| 作者单位： | 1. 苏州大学附属第二医院, 江苏 苏州 215004;2. 山东省医学科学院放射医学研究所 |
| 基金项目： | 江苏省卫生计生委2014-2015年度预防医学科研项目（Y2015024），山东省自然科学基金（ZR2016YL017） |
| 摘 要： | 目的 研究不同实验条件对双着丝粒染色体自动生物剂量估算结果的影响。方法 不同方法制备染色体标本，遗传工作站自动分析双着丝粒畸变后，用本实验室拟合的"双着丝粒染色体自动分析剂量-效应曲线"进行生物剂量估算。结果 受照血液20℃室温放置50 h、滴片细胞悬液浓度、中期分裂相分散度、染色深浅、重复扫描、遗传工作站扫描灵敏度设置、玻片细胞采集部位均不影响自动生物剂量估算结果（P > 0.05）。结论 双着丝粒染色体自动分析对实验中的一般性影响因素不敏感，有利于该方法在不同实验室间的推广。 |
| 关 键 词： | 双着丝粒染色体 自动分析 生物剂量估算 影响因素 |
| 收稿时间： | 2018-03-30 |
Study on the influence factors of automatic analysis of dicentric chromosomes for biodosimetry |
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| FENG Junchao,ZHANG Lin,DAI Hong,LIU Yulong,BIAN Huahui,CHEN Weibo,WANG Youyou,MA Ya,LIU Wei. Study on the influence factors of automatic analysis of dicentric chromosomes for biodosimetry[J]. Chinese Journal of Radiological Health, 2018, 27(4): 289-293. DOI: 10.13491/j.issn.1004-714x.2018.04.001 | |
| Authors: | FENG Junchao ZHANG Lin DAI Hong LIU Yulong BIAN Huahui CHEN Weibo WANG Youyou MA Ya LIU Wei |
| Affiliation: | 1. The Second Affiliated Hospital of Soochow University, Suzhou 215004 China;2. Institute of Radiation Medicine, Shandong Academy of Medical Sciences |
| Abstract: | Objective To study the influences of different experimental conditions on the results of automatic analysis of dicentric chromosomes for biodosimetry. Methods The dose effect curve of "automatic analysis of dicentric chromosomes" fitted by our laboratory to estimate the dose of samples prepared in different conditions after the genetic workstation automatically analyzed the dicentric chromesomes. Results The results of biodosimetry are not affected by the conditions of 50 hours at 20℃ of blood placement before inoculation, different cell suspensions concentration, different degree of dispersion in the metaphases, Wright-Giemsa staining depth, repeated scanning times, sensitivity parameter of genetic workstation and distribution positions of the metaphases on the slide (P>0.05 and have no significant difference). Conclusion Automatic analysis of dicentric chromosomes is not sensitive to the general influence factors in the experiments, and is conducive to the promotion among different laboratories. |
| Keywords: | Dicentric Chromosome Automatic Analysis Biodosimetry Influence Factors |
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