SKOV3、MRTFA在卵巢癌中的表达及其对肿瘤细胞增殖、侵袭能力的影响

目的探讨SKOV3、MRTFA在卵巢癌中的表达变化及其对肿瘤细胞增殖、侵袭能力的影响。方法将2017年6月至2019年5月宁波市鄞州人民医院收治的91例卵巢癌患者作为观察对象,对卵巢癌组织内MRTFA mRNA相对表达量和临床特征关系进行分析;对卵巢癌SKOV3细胞进行培养,分为MRTFA干扰组、阴性对照组和对照组(各组具体处理因素要列出),经Transwell法测定SKOV3细胞侵袭能力,通过Western-blot法测定SKOV3细胞内MRTFA蛋白表达。结果卵巢癌组织、癌旁组织内MRTFA mRNA相对表达量分别是(1.92±0.09)、(1.08±0.14),卵巢癌组织内MRTFA mRNA相对表达量显著高于癌旁组织,差异有统计学意义(t=48.146,P=0.000);肿瘤直径<10 cm、FIGO分期Ⅰ~Ⅱ期、中高分化、无淋巴结转移的患者卵巢癌组织内MRTFA mRNA相对表达量分别低于肿瘤直径≥10 cm、低分化、FIGO分期Ⅲ~Ⅳ期及淋巴结转移卵巢癌患者(P<0.05)。MRTFA干扰组卵巢癌SKOV3细胞培养24、48、72及96 h后吸光度值均低于对照组及阴性对照组(P<0.05);MRTFA干扰组的卵巢癌SKOV3细胞培养24 h后侵袭细胞数(86.72±4.69)低于阴性对照组(121.89±3.65)和对照组(117.10±4.41),差异有统计学意义(P<0.05)。结论MRTFA在卵巢癌中表达异常增高,对MRTFA蛋白表达进行特异性抑制,能阻断SKOV3细胞增殖及侵袭,其作用机制可能和SRF蛋白诱导上皮间充质的转化过程存在相关性。

Expression changes of SKOV3 and MRTFA in ovarian cancer and their effects on tumor cell proliferation and invasion

Objective To investigate the expression changes of SKOV3 and MRTFA in ovarian cancer and their effects on tumor cell proliferation and invasion.Methods 91 cases of patients with ovarian cancer admitted to our hospital from Jun.2017 to May.2019 were enrolled in this study.The relationship between MRTFA mRNA expression and clinical characteristics in ovarian cancer tissues was analyzed.The ovarian cancer SKOV3 cells were cultured and grouped,and the proliferation activity of ovarian cancer SKOV3 cells was detected by MTT.The invasive ability of SKOV3 cells was determined by Transwell method,and the expression of MRTFA protein in SKOV3 cells was determined by Western blot.Results The relative expression of MRTFA mRNA in ovarian cancer tissues was significantly higher than that in adjacent tissues(P<0.05).The relative expression of MRTFA mRNA in ovarian cancer tissues with tumor diameter<10 cm,FIGO stage I-II stage,moderately high differentiation and no lymph node metastasis was lower than that with tumor diameter≥10cm,FIGO stage III-IV stage,poor differentiation,lymph node metastasis in patients with ovarian cancer(P<0.05).The absorbance values of ovarian cancer SKOV3 cells in MRTFA intervention group were lower than those in the control group and the negative control group at 24 h,48 h,72 h and 96 h(P<0.05).Conclusions The expression of MRTFA is abnormally increased in ovarian cancer.The specific inhibition of MRTFA protein expression can block the proliferation and invasion of SKOV3 cells.The mechanism of action may be related to the transformation process of SRF induced epithelial mesenchyme.