极低出生体重儿支气管肺发育不良血清敏感标志物的研究

目的:通过对极低出生体重(very low birth weight,VLBW)患儿血清的可溶性晚期糖基化终产物受体(soluble receptor for advanced glycation end products,sRAGE)?内源性分泌型RAGE(endogenous secretory RAGE,esRAGE)?肺表面活性蛋白D(surfactant protein D,SP-D)?Clara细胞蛋白16(clara cell 16 kD protein,CC-16)等血清标志物水平与支气管肺发育不良(bronchopulmonary dysplasia,BPD)的相关性研究,以期找到早期评估BPD发生可能性及严重程度的敏感指标?方法:选取极低出生体重儿60例,在生后48 h内留取血清,ELISA法测定sRAGE?esRAGE?SP-D?CC-16等血清标志物水平,同时记录患儿BPD发生情况及程度,统计学分析血清学指标与BPD发生及程度的相关性?结果:①与非BPD组患儿相比,BPD组患儿血清sRAGE?esRAGE水平明显增高(P均 < 0.01);而SP-D?CC-16水平差异无统计学意义(P均 > 0.05);②BPD患儿中,sRAGE?esRAGE在轻度?中度及重度组间差异也有统计学意义;③血清sRAGE?esRAGE水平与患儿胎龄没有相关性?结论:血清sRAGE?esRAGE可以作为VLBW患儿发生BPD及严重程度的一个早期评估指标?

Research on serum biomarkers in very low birth weight infants with bronchopulmonary dysplasia

Objective:We aimed to explore sensitive serum biomarkers for the early evaluation of the occurrence and severity of bronchopulmonary dysplasia (BPD)by investigating the correlation between BPD and serum biomarkers including serum soluble receptor for advanced glycation end products (sRAGE)?endogenous secretory receptor for advanced glycation end products(esRAGE)?surfactant protein D(SP-D)and Clara cell 16 KD protein(CC-16)in the very low birth weight infants(VLBW). Methods The levels of sRAGE,esRAGE,SP-D and CC-16 in serum in 60 VLBW infants were detected by ELISA within 48 hours after birth.Meanwhile,we recorded their occurrence and valued the severity of BPD.Then we used statistical methods to analyze the correlation between serum biomarkers and the incidence and degree of BPD. Results:Compared with the normal control group,the levels of serum sRAGE and esRAGE increased significantly(P < 0.01)in the BPD group,while there was no significant difference in SP-D and CC-16 levels (P > 0.05).Additionally,the serum levels of sRAGE and esRAGE also showed statistic significance among the mild,moderate and severe BPD groups. Serum levels of sRAGE and esRAGE were not associated with gestational age. Conclusion:Serum levels of sRAGE and esRAGE can be early serum biomarkers for the occurrence and severity of BPD in VLBW infants.