超临界萃取蛇床子中香豆素类化合物的工艺优选及成分分离的研究

目的:对超临界CO₂萃取分离蛇床子中香豆素类成分进行研究。方法:采用正交试验设计方法,以提取物中总香豆素含量为考察指标,对总香豆素提取条件进行优化,同时采用重结晶法分离得到晶体并鉴定其化学结构。结果:最优萃取工艺为萃取压力25 MPa,萃取温度50℃,分离釜压力6.5 MPa,分离釜温度60℃。结论:萃取压力、萃取温度、分离温度、分离压力对实验指标有非常显著性影响;用重结晶方法从蛇床子中分得2个结晶,采用UV,IR,MS,NMR等方法,鉴定其为甲氧基欧芹素(蛇床子素,osthol)和欧前胡素(imperatorin)。

Study on extraction of coumarins in Cnidium monieri by supercritical CO2 and separation of compositions analysis

OBJECTIVE: To analyze the chemical constituents of supercritical carbon dioxide extraction products from Cnidium monieri. METHOD: Four-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. The chemical constituents were separated and identified by recrystalization. RESULT: Optimum extraction process was established: 25 MPa as extraction pressure, 50 degrees C as extraction temperature, 6.5 MPa as separation pressure and 60 degrees C as separation temperature. CONCLUSION: Changes in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably. The two kinds of chemical constituents were separated by recrystallization from C. monieri and identified by the methods of UV, IR, MS, NMR.