| 紫杉醇脂质纳米粒子对KB、SKOV3细胞的抑制作用 |
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| 引用本文： | 王宗站,许玉杰,汪梅花,刘敏,李智慧,翁婉雯,张刘珍.紫杉醇脂质纳米粒子对KB、SKOV3细胞的抑制作用[J].肿瘤,2008,28(11). |
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| 作者姓名： | 王宗站 许玉杰 汪梅花 刘敏 李智慧 翁婉雯 张刘珍 |
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| 作者单位： | 苏州大学放射医学与公共卫生学院基础核医学教研室,苏州,215123 |
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| 基金项目： | 国家自然科学基金，江苏省高校自然科学基金 |
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| 摘 要： | 目的:研究并分析新型紫杉醇脂质纳米粒子(paclitaxel nanoparlicle,TAX-NLC)对KB、SKOV3细胞的抑制作用,为研制新型肿瘤靶向治疗药物提供实验依据.方法:激光共聚焦显微镜动态观察TAX-NLC进入细胞的过程,半定量测定TAX-NLC在细胞内的浓度;MTT法测定TAX-NLC对KB、SKOV3细胞增殖的抑制率;锥虫蓝拒染法计数TAX-NLC作用后的活细胞数,绘制细胞生长曲线.结果:激光共聚焦显微镜动态观察发现细胞内荧光强度逐渐增加,约2 h时达到饱和,胞内外荧光强度差达20～40倍;MTT结果表明,TAX-NLC、紫杉醇(paclitaxel,TAX)对细胞的生长均有抑制作用,并且存在时间和剂量的依赖关系,用药后72 h后抑制率最高达92%;细胞生长曲线显示,TAX-NLC、TAX对SKOV3、KB细胞有明显的抑制作用,前者的作用强于后者.结论:TAX-NLC可能是通过主动转运将紫杉醇载带入细胞内,并在细胞内浓聚,对KB、SKOV3细胞的生长具有较强的抑制作用;其可能是一个有前途的新型肿瘤靶向治疗药物.
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| 关 键 词： | 肿瘤 实验性 细胞培养技术 紫杉醇纳米粒子 |
The inhibitory effects of TAX-NLC on the growth of KB and SKOV3 cells |
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| WANG Zong-zhan,XU Yu-jie,WANG Mei-hua,LIU Min,LI Zhi-hui,WENG Wan-wen,ZHANG Liu-zhen.The inhibitory effects of TAX-NLC on the growth of KB and SKOV3 cells[J].Tumor,2008,28(11). |
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| Authors: | WANG Zong-zhan XU Yu-jie WANG Mei-hua LIU Min LI Zhi-hui WENG Wan-wen ZHANG Liu-zhen |
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| Institution: | WANG Zong-zhan,XU Yu-jie,WANG Mei-hua,LIU Min,LI Zhi-hui,WENG Wan-wen,ZHANG Liu-zhen(Department of Basic Nuclear Medicine,School of Radiation Medicine , Public Health,Soochow University,Suzhou 215123,China) |
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| Abstract: | Objective:To study and analysis the inhibitory effects of a new type paclitaxel-nanoparticle(TAX-NLC) on KB and SKOV3 cells and provide experimental evidence for developing a novel tumor-targeted pharmaceutical agent.Methods:The dynamic process of TAX-NLC into tumor cells was observed by laser confocal microscopy and the intracellular concentration of TAX-NLC was determined semi-quantitatively.The inhibitory effects of TAX-NLC on the proliferations of KB and SKOV3 cells were measured by MTT assay.The viable... |
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| Keywords: | Neoplasms experimental Cell culture techniques Paclitaxel nanoparticle |
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