|
|||||
|
|
| 姜黄素抑制TNF-α诱导的BMEC与白细胞的粘附 | |
| 引用本文： | 张丽,梁中琴,顾振纶. 姜黄素抑制TNF-α诱导的BMEC与白细胞的粘附[J]. 中国药理学通报, 2008, 24(1): 63-67 |
| 作者姓名： | 张丽 梁中琴 顾振纶 |
| 作者单位： | 苏州大学医学院药理学教研室,苏州大学衰老和神经疾病实验室,苏州中药研究所,江苏,苏州,215123 |
| 摘 要： | 目的研究姜黄素(curcumin)对脑微血管内皮细胞(brain microvascular endothelium cell,BMEC)和白细胞粘附的影响。方法采用髓过氧化酶法测定经肿瘤坏死因子(tumor necrosis factor-α,TNF-α)刺激的BMEC对白细胞的粘附;以RT-PCR和Western blot方法检测在TNF-α作用下BMEC表面ICAM-1表达。结果经TNF-α刺激的BMEC明显增加其与白细胞的粘附反应;curcumin(12.5~100mg.L-1)可剂量依赖性地抑制TNF-α所诱导的BMEC与白细胞的粘附;在TNF-α刺激前30min加入curcumin可抑制BMEC的ICAM-1表达。结论curcumin对TNF-α所致的BMEC损伤具有保护作用,该作用可能是curcumin下调ICAM-1表达,抑制BMEC与白细胞的粘附,从而保护内皮细胞免受损伤。 |
| 关 键 词： | 姜黄素 脑微血管内皮细胞 白细胞 细胞间粘附分子-1 |
| 文章编号： | 1001-1978（2008）01-0063-05 |
| 收稿时间： | 2007-07-24 |
| 修稿时间： | 2007-09-20 |
Curcumin inhibits the adherence of leukocytes to BMECs induced by TNF-α in vitro |
|
| ZHANG Li,LIANG Zhong-qin,GU Zhen-lun. Curcumin inhibits the adherence of leukocytes to BMECs induced by TNF-α in vitro[J]. Chinese Pharmacological Bulletin, 2008, 24(1): 63-67 | |
| Authors: | ZHANG Li LIANG Zhong-qin GU Zhen-lun |
| Abstract: | Aim To investigate the effects of curcumin on the adherence between isolated rat brain microvascular endothelial cells(BMECs)and leukocytes induced by TNF-α in vitro.Methods The adherence ofleukocytes to BMECs and the effects of curcumin were determined by myeloperoxidase.The expression of ICAM-1 was detected by RT-PCR and immunoblotting.Results Curcumin inhibited the TNF-α stimulated adherence of leukocytes to BMECs.Pretreament of curcumin also inhibited TNF-α-induced increases in the mRNA and protein levels of ICAM-1 in BMECs.Conclusions Curcumin could protect the endothelial cells against damage caused by TNF-α.The protective effects of curcumin may be mediated through downregulation of the expression of ICAM-1. |
| Keywords: | curcumin brain microvascular endothelial cells leukocytes ICAM-1 |
| 本文献已被 万方数据 等数据库收录！ | |