| 反相高效液相色谱法测定益气强身胶囊中人参皂苷Re和人参皂苷Rg1的含量 |
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| 引用本文： | 肖金芳,周建红. 反相高效液相色谱法测定益气强身胶囊中人参皂苷Re和人参皂苷Rg1的含量[J]. 中南药学, 2010, 8(11): 842-844. DOI: 10.3969/j.issn.1672-2981.2010.11.014 |
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| 作者姓名： | 肖金芳 周建红 |
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| 作者单位： | [1]湖南省娄底市第二人民医院,湖南娄底417000 [2]湖南省娄底市第一人民医院,湖南娄底417000 |
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| 摘 要： | 目的 建立RP-HPLC测定益气强身胶囊中人参皂苷Re、人参皂苷Rg1含量的方法.方法 采用Thermo C18柱,以乙腈-水（19：81）为流动相,检测波长：203 nm,流速：1.0 mL·min-1,柱温：26℃.结果 人参皂苷Re在0.411 2～2.056 μg与峰面积线性关系良好,r=0.999 7,平均加样回收率为99.3%;人参皂苷Rg1在0.494 4～2.472 μg与峰面积线性关系良好,r=0.999 8,平均加样回收率为99.6%.结论 本方法操作简便,方法可靠,结果准确、灵敏度高,重现性好,可作为该产品质量控制的方法.
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| 关 键 词： | 高效液相色谱法 益气强身胶囊 人参皂苷Re 人参皂苷Rg1 |
Determination of ginsenoside Re and ginsenoside Rg1 in Yiqi Qiangshen Jiaonang by RP-HPLC |
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| XIAO Jin-fang,ZHOU Jian-hong. Determination of ginsenoside Re and ginsenoside Rg1 in Yiqi Qiangshen Jiaonang by RP-HPLC[J]. Central South Pharmacy, 2010, 8(11): 842-844. DOI: 10.3969/j.issn.1672-2981.2010.11.014 |
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| Authors: | XIAO Jin-fang ZHOU Jian-hong |
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| Affiliation: | 1.Second People’s Hospital of Loudi,Loudi Hunan 417000;2.First People’s Hospital of Loudi,Loudi Hunan 417000) |
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| Abstract: | Objective To establish an RP-HPLC method for the determination of ginsenoside Re and ginsenoside Rg1 in Yiqi Qiangshen Jiaonang.Methods Thermo C18 column was used.The mobile phase was acetonitrile-water（19∶81）.The detection wavelength was 203 nm.The flow rate was 1.0 mL·min-1.Results The linear range of Ginsenoside Re was within 0.411 2-2.056 μg,r=0.999 7,and the average recovery was 99.3%.The linear range of Ginsenoside Rg1 was within 0.494 4-2.472 μg,r=0.999 8,and the average recovery was 99.6%.Conclusion This method is repeatable,flexible and highly sensitive.It can be used for the quality control of Yiqi Qiangshen Jiaonang. |
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| Keywords: | HPLC Yiqi Qiangshen Jiaonang ginsenoside Re ginsenoside Rg1 |
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