VEGF在乳腺癌组织与血清中的表达及临床意义

目的探讨血管内皮生长因子（VEGF）与乳腺癌发生、发展及淋巴结转移的关系。方法免疫组化方法测定不同组织学类型乳腺癌组织中VEGF水平；用双抗体夹心酶联免疫吸附法（ELIsA）测定血清中VEGF水平。结果1）VEGF表达阳性率随着乳腺癌组织学分级增高而逐渐增高，淋巴结转移的乳腺癌组VEGF阳性表达率为92．86％（26／28），无淋巴结转移的乳腺癌组VEGF阳性表达率为81．82％（18／22）；2）正常对照组和良性乳腺癌组血清VEGF水平比较，两组问差异无统计学意义（P〉0．05）；正常对照组与恶性乳腺癌组进行比较，差异有统计学意义（P〈0．01）；良性乳腺癌组与恶性乳腺癌组比较，其VEGF的浓度较恶性乳腺癌患者低，差异有统计学意义（P〈0．01）。结论VEGF在乳腺癌中高表达，提示其可能与乳腺癌的恶性进展有关；VEGF与乳腺癌淋巴结转移有关，检测VEGF含量对预测乳腺癌淋巴结转移具有重要意义。

Expression of VEGF in Breast Cancer Tissue and its Clinical Significance

Objective To investigate the expressions of vascular endothelial growth factor(VEGF) in serum and breast cancer tissues,and to explore their relationships with the occurrence,development and lymph node metastasis of the cancer.Methods VEGF expression in the breast cancer tissue of different histological types was determined by immunohistochemistry,and VEGF level in serum was detected by ELISA.Results The positive rate of VEGF expression was increased with the progress of the histological grade of the breast cancer.The positive rate of VEGF in the lymph node metastasis group was 92.86%(26/28),while that in the non lymph node metastasis group was 81.82%(18/22).The serum VEGF level in the lymph node metastasis group was significantly higher than that in the non lymph node metastasis group(P<0.05).The serum VEGF level was not significantly different between the normal control group and the benign breast cancer group(P>0.05),while that in the malignant breast cancer group was significantly higher than those in the other two groups(P<0.01).Conclusions VEGF is highly expressed in breast cancer.It may correlate with the malignancy and node metastasis of the cancer.Detection of VEGF is of important significance in predicting node metastasis of breast cancer.