干燥法长期保存角膜板层移植1267例临床研究

目的：评价干燥脱水法长期保存角膜片在板层治疗性角膜移植术中的效果。方法：保存角膜片选自我院眼库干燥保存符合临床标准、复水透明的完整角膜片，临床病例选自我院角膜病组1976年8月～1999年12月期间行板层治疗性角膜移植患者1267例，从手术前后视力比较、原发病灶控制、植片存活情况进行临床效果综合分析。结果：保存角膜片复水透明合格率为94．9％，临床治愈率：感染性角膜溃疡／穿孔88．8％，血管翳性全角膜白斑98．3％。化学及热烧伤(穿孔，睑球角粘连等)92．1％，圆锥角膜及急性圆锥角膜99．5％，角膜变性(边缘性及前基质层)99．0％。其他88．9％，平均总治愈率93．5％。结论：1．对各种严重的角膜感染、药物治疗无法控制的病人，治疗性角膜移植术是唯一有效的方法。2．采用无水氯化钙一硅胶干燥脱水长期保存的供体角膜能为临床随时提供角膜材料，是一项简单易行、便于推广应用的成功率很高的保存方法。

The clinical study of 1267 cases therapeutic keratoplasty with grafts preserved in long- time dehydration

Objective:To evaluate the effects of long-time dehydrated preservation of donor corneal grafts. Methods:1267 cases of therapeutic keratoplasty were performed with long-time dehydrated preservation of donor corneal grafts from Xian Eye Bank.The preoperative and postoperative visual acuities,controlling of original focus,revival scene of donor corneal grafts were analyzed. Results:The transparent rate of donor corneal grafts after re-hydration was 94.9%.The clinical effective rates in infective corneal ulcer/perforation,pannus totalis and corneal opacity,chemical burn and heat burn (perforation corneoblepharon),keratoconus and acute keratoconus,corneal degeneration(marginal,shallow of stroma) and other diseases were all over 88%,mean 93.5%. Conclusions:Long-time dehydrated corneal preservation in water-free calcium chloride-silica gel can provide clinical supply of corneal materials whenever necessary,it is an easy and simple,highly applicable method.