全反式维甲酸联合顺铂在诱导人头颈癌 TCA8113细胞凋亡中的作用

目的：研究全反式维甲酸（ATRA）与顺铂对人头颈癌 TCA8113细胞凋亡的影响。方法 ATRA 和顺铂单独及联合作用于 TCA8113细胞，采用 MTT 法检测细胞生长，并观察细胞形态变化；使用流式细胞仪检测细胞周期及凋亡；通过 western blot 实验检测 caspase-3表达情况。结果 ATRA 作用于 TCA8113细胞后，细胞生长明显受到抑制，细胞形态发生改变，细胞凋亡比例明显增加，caspase-3蛋白表达下调，ATRA 与顺铂联合作用于肿瘤细胞后，其抑癌作用加强。结论 ATRA 联合顺铂对人头颈癌 TCA8113细胞具有协同抑癌作用。

Effect of All-trans Retinoic Acid Compared with Cisplatin in Inducing Apoptosis of TCA8113 Cell Lines

Objective To observe the effect of all-trans retinoic acid(ATRA)and cisplatin on the proliferation of human head and neck cancer TCA8113 cells. Methods TCA8113 cells were treated with ATRA and(or)cispl-atin respectively. Cell proliferation was detected by the method of MTT. Cell cycle was detected by flow cytometry. Results The growth of the cells treated with ATRA was inhibited obviously and the morphology of the cells were changed. The apoptosis rate of TCA8113 cells was increased gradually and the effect was enhanced when combined ATRA with cisplatin. Conclusion ATRA could inhibit the proliferation of human head and neck cancer TCA8113 cells and the inhibitory effect was synergistic when ATRA combined with cisplatin.