膀胱出口部分梗阻早期结缔组织生长因子的表达及意义

目的 研究结缔组织生长因子(CTGF)在大鼠膀胱出口部分梗阻(B00)致膀胱重塑时早期各时相在逼尿肌中的表达.方法 Wistar大鼠36只随机分为3组:梗阻7d组、梗阻14d组及对照组,每组12只.按设计,各时段获取膀胱标本,测量其重量及最大膀胱容量.用逆转录-聚合酶链反应(RT-PCR)半定量法测定膀胱逼尿肌的CTGF mRNA.CTGF相关蛋白定位、定性诊断实验用免疫组织化学法.结果 梗阻14 d组(B002组)、梗阻7 d组(B001组)的CTGFmRNA相对峰值分别为0.99±0.58、0.84±0.12.两组比较差异有统计学意义(P＜0.05).B002组、B001组膀胱重量分别为(241.08±4.36)、(237.08±6.02)g,均高于对照组(158.50±4.62)g(P＜0.05).B002组、B001组膀胱容量分别为(0.82±0.03)、(0.74±0.03)ml,均高于对照组(0.62±0.02)ml(P＜0.05).免疫组织化学结果提示:B001组(3/12)及B002组的标本部分或全部免疫染色,显色区分布在黏膜下固有层或平滑肌肌束间.对照组无1例染色.结论 CTGF是PB00膀胱纤维化早期过程中重要的调节因子.

Expression of connective tissue growth factor in the rat bladder hyperplasia secondary to partial bladder outlet obstruction

Objective To investigate the gene expression profile of connective tissue growth factor (CTGF) in rat urinary bladder during growth in response to partial bladder outlet obstruction (PBOO).Methods Thirty-six healthy male Wistar rats were randomly divided into 3 groups:B001 group (n = 12),B002 group (n = 12) and the control group (n = 12).The bladder necks were partially ligated to form B00 animal model in obstruction group.On the postoperative day 7 and 14 the bladders were resected separately in every group,and the weight and volume of the bladders were measured.The mRNA expression of CTGF was detected semiquantitatively by using reverse transciption-polymerase chain reaction (RT-PCR).The localization of CTGF proteins was performed by immunohistechemical staining assay.Results The bladder weights in B001 group,B002 group and control group were (241.08±4.36) g,(237.08±6.02) g and ( 158.50±4.62) g,respectively (P＜0.05 ),and the bladder volumes were (0.82±0.03 ) ml,(0.74±0.03) ml and (0.62±0.02) ml,respectively (P＜0.05 ).The mRNA expression of CTGF was significandy upregulated in B002 group (0.99±0.58) compared to that in B001 group (0.84±0.12) (P＜ 0.05).Immunostaining for CTGF was seen in all of BOO2 group and part of BOO1 group (3/12) ,which was mainly located within both lamina propria layers and surrounding the smooth muscle fascicles.Conclusion These data strongly indicate the involvement of CTGF in bladder wall remodeling as a result of the outlet obstruction.CTGF is involved in the progression of early bladder fibrosis following PB00.