| 地高辛标记探针检测重组双功能水蛭素中的DNA残留量 |
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| 引用本文： | 蔡旭,马端,刘康达,宋后燕. 地高辛标记探针检测重组双功能水蛭素中的DNA残留量[J]. 药物生物技术, 2004, 11(6): 392-394 |
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| 作者姓名： | 蔡旭 马端 刘康达 宋后燕 |
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| 作者单位： | 1. 复旦大学分子医学教育部重点实验室,上海,200032
2. 复旦大学附属中山医院中心实验室,上海,200032 |
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| 摘 要： | 为检测注射用重组双功能水蛭素半成品中的DNA残留量，应用地高辛标记重组双功能水蛭素工程菌DNA，并以此为DNA探针进行点杂交，同时做特异性及灵敏度实验，结果表明，该方法检测最低限值可达1pg,且重复性好，特异性强，操作较简便，可用于双功能水蛭素制备工艺的质控及半成品的检定。
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| 关 键 词： | 地高辛标记探针 点杂交法 水蛭素 DNA残留量 |
| 文章编号： | 1005-8915(2004)06-0392-03 |
Analysis for Residual DNA in Recombinant Double Functions Hirudin Using Digoxin Labeled Probe |
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| CAI Xu ,MA Duan ,LIU Kang-da ,SONG Hou-yan. Analysis for Residual DNA in Recombinant Double Functions Hirudin Using Digoxin Labeled Probe[J]. Pharmaceutical Biotechnology, 2004, 11(6): 392-394 |
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| Authors: | CAI Xu MA Duan LIU Kang-da SONG Hou-yan |
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| Affiliation: | CAI Xu 1,MA Duan 1,LIU Kang-da 2,SONG Hou-yan 1 |
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| Abstract: | DNA of engineering bacteria of recombinant double functions hirudin were labeled by Digoxin and used as probe for dot hybridization to detect residual DNA in semi-finished products of recombinant double functions hirudin for injection. At the same time, specificity and sensitivity experiments were performed. It was proved by experimental results that the lowest volume of detection was up to 1 pg. This method had good repeatability and specificity, simple operation, and was competent for quality control in preparation technique of recombinant double functions hirudin and assay for semi-finished products. |
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| Keywords: | Digoxin labeled probe Dot hybridization Hirudin Residual DNA |
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