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| 蒽酮硫酸法测定牛蒡利咽粥中多糖含量 | |
| 引用本文： | 惠秋沙. 蒽酮硫酸法测定牛蒡利咽粥中多糖含量[J]. 北方药学, 2011, 8(8): 4-5 |
| 作者姓名： | 惠秋沙 |
| 作者单位： | 山东中医药大学,济南,250014 |
| 摘 要： | 目的：测定牛蒡利咽粥中多糖含量。方法：采用蒽酮-硫酸法,以葡萄糖为对照品,于625nm波长处测其吸光度,测定总多糖的含量。结果：葡萄糖质量浓度在0.02～0.14mg·mL-1范围内与吸收度呈良好的线性关系;平均回收率为99.90%,RSD=0.20%;测得的总多糖平均含量为21.69mg·g-1。结论：采用蒽酮-硫酸法测定牛蒡利咽粥中总多糖含量,快速准确,稳定性好,可作为该制剂总多糖含量测定方法。 |
| 关 键 词： | 牛蒡利咽粥 蒽酮硫酸法 多糖含量测定 |
Assay determination of polysaccharides in Burdock Liyanporridge by Anthrone-sulfuric acid method |
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| Hui Qiusha. Assay determination of polysaccharides in Burdock Liyanporridge by Anthrone-sulfuric acid method[J]. BEIFANG YAOXUE, 2011, 8(8): 4-5 | |
| Authors: | Hui Qiusha |
| Affiliation: | Hui Qiusha(Shandong University of Traditional Chinese Medicine,Jinan,250014) |
| Abstract: | Objective：To determine the assay of polysaccharide in Burdock Liyan porridge.Methods：The assay of polysaccharides was measured with UV-Vis method by the anthrone-sulfuric acid using glucose as the standard in the 625nm wavelength.Results：The calibration curve was linear within the range of 0.02～0.14mg·ml-1 for glucose.The average recovery was 99.90% with RSD 0.20%.The average assay of polysaccharide is 21.69mg·g-1.Conclusion：The method was accurate,stable and suitable for the quality control of Burdock Liyan porridge. |
| Keywords: | Burdock Liyan porridge Anthrone sulfuric acid method Content determination of Polysaccharide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |