| 应用酶联免疫吸附试验检测猪囊虫病特异抗体的研究 |
| |
| 引用本文： | 翟春生,王永山,施正良. 应用酶联免疫吸附试验检测猪囊虫病特异抗体的研究[J]. 中国人兽共患病杂志, 1990, 6(6): 43-46 |
| |
| 作者姓名： | 翟春生 王永山 施正良 |
| |
| 作者单位： | 南京军区军事医学研究所,南京军区军事医学研究所,南京军区军事医学研究所 |
| |
| 摘 要： | 用猪囊虫粗抗原、B 抗原、等电聚焦技术分离的抗原组份和特异单克隆抗体亲和层析抗原,以ELSA间接法检测猪囊虫病血清抗体,结果特异性均不理想。应用单克隆抗体分析查明,在某些囊虫抗原分子上既有特异性抗原位点,又有非特异性位点。用特异单克隆抗体以ELISA抑制法检测猪囊虫病血清抗体,结果完全消除了假阳性反应,病猪血清检出率为86.41％(89/103)。
|
| 关 键 词： | 猪囊虫病 单克隆抗体 酶联免疫吸附试验 |
| 收稿时间： | 1990-06-20 |
A Study on the Detection of Specific Antibodies of Swine Cysticercosis with ELISA |
| |
| Zhai Chunsheng. A Study on the Detection of Specific Antibodies of Swine Cysticercosis with ELISA[J]. Chinese Journal of Zoonoses, 1990, 6(6): 43-46 |
| |
| Authors: | Zhai Chunsheng |
| |
| Affiliation: | Zhai Chunsheng |
| |
| Abstract: | The antigens of Cysticercus cellulosae including crude antigen, B- antigen, purified by isoelectric separation and by affinity chromatography with monoclonal antibodies (McAbs) were used in detection of antibodies of C. cellulosae in swine serum with indirect ELISA. Results showed that the specificity is not perfect. The analysis of the antigens with McAbsrevealed that some antigens contained both specific and non-specific epitopes. The antibodies of C. cellulosae were detected by inhibit ELISA with HRP- labeled McAbsin swine serum. The results indicated that the false positive reaction could be eliminated completely,and detectable proportoin of tested sera from swine with cysticercosis was 86. 41% (89/ 103). |
| |
| Keywords: | Swine cysticercosis Monoclonal antibody ELISA Specific antibody Detection. |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国人兽共患病杂志》浏览原始摘要信息 |
|
点击此处可从《中国人兽共患病杂志》下载全文 |
|
