广西巴马长寿老人肾虚证候与ACE基因多态性的相关性研究

目的探讨广西巴马长寿地区老年人肾虚证候与血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性的关系。方法采用问卷调查并采血进行聚合酶链反应(PCR)、单链构象多态性分析(SSCP)与直接测序技术检验确认,研究百岁组(≥100岁)27名、90岁组(90~99岁)56名、老年组(60~89岁)114名老人的肾虚证候及其与ACE基因I/D多态性的关系,以79例60~70岁自然死亡人群为对照。结果D/D型老年人肾虚症状最少,I/D型老年人次之,I/I型老年人肾虚症状最多。在ACE基因I/D多态基因型中,百岁组I/I、I/D、D/D型频率分别为37.04%(10/27)、33.33%(9/27)和29.63%(8/27),I和D等位基因频率分别为53.70%(29/54)和46.30%(25/54)。百岁组D/D基因型频率和D型等位基因频率高于老年组及对照组(P0.05,P0.01),但与90岁组比较,无论是I/I、I/D或D/D基因型,所占百分率均比较接近,差异无统计学意义(P0.05)。D/D基因型经SSCP证实全部相同,无差别。直接测序结果再次验证ACE(I/D)多态性判断准确。结论老年肾虚证候与ACE基因I/D多态性及长寿密切相关,同时也应考虑到长寿受多种因素影响。

Relationship between Shen-deficiency Syndrome and Polymorphism of Angiotensin Converting Enzyme Gene in Longevous Elders in Bama Area of Guangxi Municipality

ObjectiveTo explore the relationship between Shen-deficiency syndrome(SDS) and the insert/deficit(I/D) polymorphism types(PMTs) of angiotensin converting enzyme(ACE) gene in longevous elders in Bama area of Guangxi municipality.MethodsThe investigation on 27 centenarians(Group A),56 elders of 90-99 years old(Group B) and 114 of 60-89 years old(Group C) was carried out by questionnaire,and the polymorphism was detected using polymerize chain reaction detection,single strand conformation polymorphism(SSCP) an...